ArticleJournal of bacteriology2009
In Escherichia coli, MreB and FtsZ direct the synthesis of lateral cell wall via independent pathways that require PBP 2.
Article in Journal of bacteriology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 53 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
53 citing papers in PubMed, 78 citations in OpenAlex.
- Alanine-scanning mutagenesis library of MreB reveals distinct roles for regulating cell shape and viability.PLoS genetics · 2026Article
- MreB: unraveling the molecular mechanisms of bacterial shape, division, and environmental adaptation.Cell communication and signaling : CCS · 2025Review
- Age-Dependent Pleomorphism inMicroorganisms · 2025Article
- Microwell-enhanced optical rapid antibiotic susceptibility testing of single bacteria.iScience · 2023Article
- Models versus pathogens: how conserved is the FtsZ in bacteria?Bioscience reports · 2023Review
- MukBEF-dependent chromosomal organization in widenedFrontiers in microbiology · 2023Article
- Cell density-dependent antibiotic tolerance to inhibition of the elongation machinery requires fully functional PBP1B.Communications biology · 2022Article
- How Does the Spatial Confinement of FtsZ to a Membrane Surface Affect Its Polymerization Properties and Function?Frontiers in microbiology · 2022Review
- A Dynamic Network of Proteins Facilitate Cell Envelope Biogenesis in Gram-Negative Bacteria.International journal of molecular sciences · 2021Review
- Peptidoglycan: Structure, Synthesis, and Regulation.EcoSal Plus · 2021Article
- Elongation at Midcell in Preparation of Cell Division Requires FtsZ, but Not MreB nor PBP2 inFrontiers in microbiology · 2021Article
- Antimicrobial and Antivirulence Impacts of Phenolics on Salmonella Enterica Serovar Typhimurium.Antibiotics (Basel, Switzerland) · 2020Article
- Morphology engineering: a new strategy to construct microbial cell factories.World journal of microbiology & biotechnology · 2020Review
- Flotillin-mediated membrane fluidity controls peptidoglycan synthesis and MreB movement.eLife · 2020Article
- Enhanced synthesis of alginate oligosaccharides in3 Biotech · 2019Article
- Status of Targeting MreB for the Development of Antibiotics.Frontiers in chemistry · 2019Review
- Colistin causes profound morphological alteration but minimal cytoplasmic membrane perforation in populations of Escherichia coli and Pseudomonas aeruginosa.Archives of microbiology · 2018Article
- Heterogeneous localisation of membrane proteins in Staphylococcus aureus.Scientific reports · 2018Article
- Bacterial Cell Size: Multifactorial and Multifaceted.Annual review of microbiology · 2017Review
- Roles of the Essential Protein FtsA in Cell Growth and Division in Streptococcus pneumoniae.Journal of bacteriology · 2017Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
In Escherichia coli, the cytoplasmic proteins MreB and FtsZ play crucial roles in ensuring that new muropeptide subunits are inserted into the cell wall in a spatially correct way during elongation and division. In particular, to retain a constant diameter and overall shape, new material must be inserted into the wall uniformly around the cell's perimeter. Current thinking is that MreB accomplishes this feat through intermediary proteins that tether peptidoglycan synthases to the outer face of the inner membrane. We tested this idea in E. coli by using a DD-carboxypeptidase mutant that accumulates pentapeptides in its peptidoglycan, allowing us to visualize new muropeptide incorporation. Surprisingly, inhibiting MreB with the antibiotic A22 did not result in uneven insertion of new wall, although the cells bulged and lost their rod shapes. Instead, uneven (clustered) incorporation occurred only if MreB and FtsZ were inactivated simultaneously, providing the first evidence in E. coli that FtsZ can direct murein incorporation into the lateral cell wall independently of MreB. Inhibiting penicillin binding protein 2 (PBP 2) alone produced the same clustered phenotype, implying that MreB and FtsZ tether peptidoglycan synthases via a common mechanism that includes PBP 2. However, cell shape was determined only by the presence or absence of MreB and not by the even distribution of new wall material as directed by FtsZ.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.