ArticleCurrent biology : CB2009
In vivo protein architecture of the eukaryotic kinetochore with nanometer scale accuracy.
Article in Current biology : CB, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 143 papers.
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Who cites it
143 citing papers in PubMed, 185 citations in OpenAlex.
- Mitotic error correction and the spindle assembly checkpoint: a tension-filled relationship.Cell cycle (Georgetown, Tex.) · 2026Review
- Research Report on NUF2-Targeted Therapy for Digestive System Cancers: from Mechanism to Clinical Translation.Current oncology reports · 2026Review
- Article
- Sustainable integrative cell biology: CENP-C is guilty by association.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2025Article
- A coordinated kinase and phosphatase network regulates Stu2 recruitment to yeast kinetochores.The Journal of cell biology · 2025Article
- The centromere bottlebrush requires a multi-microtubule attachment.Molecular biology of the cell · 2025Article
- Kinetochores grip microtubules with directionally asymmetric strength.The Journal of cell biology · 2025Article
- <italic>Zea mays</italic> Meiotic Spindle Ultrastructure Reveals Kinetochore-Microtubule Interface and Embedded Membrane Components.Cytogenetic and genome research · 2025Article
- Architecture of native kinetochores revealed by structural studies utilizing a thermophilic yeast.Current biology : CB · 2024Article
- Architecture and flexibility of native kinetochores revealed by structural studies utilizing a thermophilic yeast.bioRxiv : the preprint server for biology · 2024Article
- Unraveling the kinetochore nanostructure in Schizosaccharomyces pombe using multi-color SMLM imaging.The Journal of cell biology · 2023Article
- Nanoscale structural organization and stoichiometry of the budding yeast kinetochore.The Journal of cell biology · 2023Article
- The importance of microtubule-dependent tension in accurate chromosome segregation.Frontiers in cell and developmental biology · 2023Review
- The Four Causes: The Functional Architecture of Centromeres and Kinetochores.Annual review of genetics · 2022Review
- Aurora B activity is promoted by cooperation between discrete localization sites in budding yeast.Molecular biology of the cell · 2022Article
- Tension can directly suppress Aurora B kinase-triggered release of kinetochore-microtubule attachments.Nature communications · 2022Article
- Shaping centromeres to resist mitotic spindle forces.Journal of cell science · 2022Article
- Kre28-Spc105 interaction is essential for Spc105 loading at the kinetochore.Open biology · 2022Article
- Role of spatial patterns and kinetochore architecture in spindle morphogenesis.Seminars in cell & developmental biology · 2021Review
- Bridgin connects the outer kinetochore to centromeric chromatin.Nature communications · 2021Article
83 more citing papers are in PubMed but not listed here.
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The kinetochore is a macromolecular protein machine [1] that links centromeric chromatin to the plus ends of one or more microtubules (MTs) and segregates chromosomes during cell division. Its core structure consists of eight multicomponent protein complexes, most of which are conserved in all eukaryotes. We use an in vivo two-color fluorescence microscopy technique to determine, for the first time, the location of these proteins along the budding yeast kinetochore axis at nanometer resolution. Together with kinetochore protein counts [2, 3], these localizations predict the 3D protein architecture of a metaphase kinetochore-microtubule attachment and provide new functional insights. We also find that the kinetochore becomes much shorter in anaphase as metaphase tension is lost. Shortening is due mainly to a decrease in the length of the Ndc80 complex, which may result either from intramolecular bending of the Ndc80 complex at the kink within the stalk region of the Ndc80-Nuf2 dimer [4, 5] or from a change in its orientation relative to the microtubule axis. Conformational changes within the Ndc80 and Mtw1 complexes may serve as mechanical cues for tension-dependent regulation of MT attachment and the spindle-assembly checkpoint. The geometry of the core structure of the budding yeast kinetochore reported here is remarkably similar to that found in mammalian kinetochores, indicating that kinetochore structure is conserved in eukaryotes with either point or regional centromeres.
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