ArticleJournal of cellular biochemistry2009
Evidence for phosphatase activity of p27SJ and its impact on the cell cycle.
Article in Journal of cellular biochemistry, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 25 citations in OpenAlex.
- Shell Matrix Protein N38 of Pinctada fucata, Inducing Vaterite Formation, Extends the DING Protein to the Mollusca World.Marine biotechnology (New York, N.Y.) · 2022Article
- DING Proteins Extend to the Extremophilic World.International journal of molecular sciences · 2021Review
- DING Protein Inhibits Transcription of HIV-1 Gene through Suppression of Phosphorylation of NF-κB p65.Journal of HIV and AIDS · 2020Article
- Human X-DING-CD4 mediates resistance to HIV-1 infection through novel paracrine-like signaling.The FEBS journal · 2015Article
- Crystal structure of the phosphate-binding protein (PBP-1) of an ABC-type phosphate transporter from Clostridium perfringens.Scientific reports · 2014Article
- Neuroprotective activity of pDING in response to HIV-1 Tat.Journal of cellular physiology · 2014Article
- DING proteins: numerous functions, elusive genes, a potential for health.Cellular and molecular life sciences : CMLS · 2013Review
- Pseudomonas DING proteins as human transcriptional regulators and HIV-1 antagonists.Virology journal · 2013Article
- Crystallization and preliminary X-ray diffraction analysis of a DING protein from Pseudomonas aeruginosa PA14.Acta crystallographica. Section F, Structural biology and crystallization communications · 2013Article
- Article
- Growth inhibition of malignant glioblastoma by DING protein.Journal of neuro-oncology · 2012Article
- The level of DING proteins is increased in HIV-infected patients: in vitro and in vivo studies.PloS one · 2012Article
- Human-Phosphate-Binding-Protein inhibits HIV-1 gene transcription and replication.Virology journal · 2011Article
- Structural insights and ab initio sequencing within the DING proteins family.Journal of synchrotron radiation · 2011Article
- Suppression of HIV-1 transcriptional elongation by a DING phosphatase.Journal of cellular biochemistry · 2011Article
- Eukaryotic DING proteins are endogenous: an immunohistological study in mouse tissues.PloS one · 2010Article
- p38SJ, a novel DINGG protein protects neuronal cells from alcohol induced injury and death.Journal of cellular physiology · 2009Article
- Identification of X-DING-CD4, a new member of human DING protein family that is secreted by HIV-1 resistant CD4(+) T cells and has anti-viral activity.Biochemical and biophysical research communications · 2009Article
Corrections and comments
- Erratum issuedErratum.2021
Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
Abstract
p27SJ, a novel protein isolated from St John's wort (Hypericum perforatum), belongs to an emerging family of DING proteins that are related to a prokaryotic phosphate-binding protein superfamily. Here we demonstrate that p27SJ exhibits phosphatase activity and that its expression in cells decreases the level of phosphorylated Erk1/2, a key protein of several signaling pathways. Treatment of p27SJ-expressing cells with phosphatase inhibitors including okadaic acid, maintained Erk1/2 in its phosphorylated form, suggesting that dephosphorylation of Erk1/2 is mediated by p27SJ. Further, expression of p27SJ affects Erk1/2 downstream regulatory targets such as STAT3 and CREB. Moreover, the level of expression of cyclin A that associates with active ERK1/2 and is regulated by CREB, was modestly reduced in p27SJ-expressing cells. Accordingly, results from in vitro kinase assays revealed a noticeable decrease in the activity of cyclin A in cells expressing p27SJ. Cell cycle analysis demonstrated dysregulation at S and G2/M phases in cells expressing p27SJ, supporting the notion that a decline in cyclin A activity by p27SJ has a biological impact on cell growth. These observations provide evidence that p27SJ alters the state of Erk1/2 phosphorylation, and impacts several biological events associated with cell growth and function.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.