ArticleTransgenic research2009
Expression and purification of an anti-Foot-and-mouth disease virus single chain variable antibody fragment in tobacco plants.
Article in Transgenic research, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
9 citing papers in PubMed.
- Recombinant Antibodies in Veterinary Medicine: An Update.Frontiers in veterinary science · 2018Review
- A hyper-thermostable α-amylase from Pyrococcus furiosus accumulates in Nicotiana tabacum as functional aggregates.BMC biotechnology · 2017Article
- Anti-infective immunoadhesins from plants.Plant biotechnology journal · 2015Review
- Foot-and-mouth disease: overview of motives of disease spread and efficacy of available vaccines.Journal of animal science and technology · 2015Article
- Influence of elastin-like polypeptide and hydrophobin on recombinant hemagglutinin accumulations in transgenic tobacco plants.PloS one · 2014Article
- Recent advances and safety issues of transgenic plant-derived vaccines.Applied microbiology and biotechnology · 2013Review
- Hydrophobin fusions for high-level transient protein expression and purification in Nicotiana benthamiana.Plant physiology · 2010Article
- Induction of protein body formation in plant leaves by elastin-like polypeptide fusions.BMC biology · 2009Article
- Plants as bioreactors: Recent developments and emerging opportunities.Biotechnology advancesReview
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Low-cost recombinant antibodies could provide a new strategy to control Foot-and-mouth disease virus (FMDV) outbreaks by passive immunization of susceptible animals. In this study, a single chain variable antibody fragment (scFv) recognizing FMDV coat protein VP1 was expressed in transgenic tobacco plants. To enhance the accumulation of scFv protein, the codon-usage of a murine hybridoma-derived scFv gene was adjusted to mimic highly expressed tobacco genes and fused to an elastin-like polypeptide (ELP) tag. This scFv-ELP fusion accumulated up to 0.8% of total soluble leaf protein in transgenic tobacco. To recover scFv-ELP protein from the leaf extract, a simple and scalable purification strategy was established. Purified scFv-ELP fusion was cleaved to separate the scFv portion. Finally, it was shown that the purified scFv proteins retained their capacity to bind the FMDV in the absence or presence of ELP fusion.
Indexed as
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.