ArticlePlant cell reports2009
Agrobacterium-mediated transformation of Fraxinus pennsylvanica hypocotyls and plant regeneration.
Article in Plant cell reports, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
9 citing papers in PubMed, 37 citations in OpenAlex.
- Perspectives of Gene Editing for the Conservation of Plant Genetic Resources.Methods in molecular biology (Clifton, N.J.) · 2026Review
- Application of Nanotechnology in Plant Genetic Engineering.International journal of molecular sciences · 2023Review
- A simple and efficient protocol for transient transformation of sliced grape berries.Protoplasma · 2023Article
- Transformation of European Ash (Plants (Basel, Switzerland) · 2021Article
- An Optimized Transformation System and Functional Test ofInternational journal of molecular sciences · 2021Article
- Agrobacterium-mediated genetic transformation and plant regeneration of the hardwood tree species Fraxinus profunda.Plant cell reports · 2014Article
- Improved Agrobacterium-mediated transformation of cowpea via sonication and vacuum infiltration.Plant cell reports · 2011Article
- Interspecific proteomic comparisons reveal ash phloem genes potentially involved in constitutive resistance to the emerald ash borer.PloS one · 2011Article
- Regeneration of transgenic plants by Agrobacterium-mediated transformation of somatic embryos of juvenile and mature Quercus robur.Plant cell reports · 2010Article
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A genetic transformation protocol for green ash (Fraxinus pennsylvanica) hypocotyl explants was developed. Green ash hypocotyls were transformed using Agrobacterium tumefaciens strain EHA105 harboring binary vector pq35GR containing the neomycin phosphotransferase (nptII) and beta-glucuronidase (GUS) fusion gene, and an enhanced green fluorescent protein gene. Pre-cultured hypocotyl explants were transformed in the presence of 100 microM acetosyringone using 90 s sonication plus 10 min vacuum-infiltration. Kanamycin at 20 mg l(-1) was used for selecting transformed cells. Adventitious shoots regenerated on Murashige and Skoog medium supplemented with 13.3 microM 6-benzylaminopurine, 4.5 microM thidiazuron, 50 mg l(-1) adenine sulfate, and 10% coconut water. GUS- and polymerase chain reaction (PCR)-positive shoots from the cut ends of hypocotyls were produced via an intermediate callus stage. Presence of the GUS and nptII genes in GUS-positive shoots were confirmed by PCR and copy number of the nptII gene in PCR-positive shoots was determined by Southern blotting. Three transgenic plantlets were acclimatized to the greenhouse. This transformation and regeneration system using hypocotyls provides a foundation for Agrobacterium-mediated transformation of green ash. Studies are underway using a construct containing the Cry8Da protein of Bacillus thuringiensis for genetic transformation of green ash.
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Registered trials
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