ArticleJournal of biomedicine & biotechnology2009
Purification and characterization of a lectin from Phaseolus vulgaris cv. (Anasazi beans).
Article in Journal of biomedicine & biotechnology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 63 citations in OpenAlex.
- Unlocking Nature's Shield: The Promising Potential of CRISPRa in Amplifying Antimicrobial Peptide Expression in Common Bean (ACS omega · 2025Article
- Partial Characterization of Lectins Purified from the Surco and Vara (Furrow and Rod) Varieties of BlackMolecules (Basel, Switzerland) · 2022Article
- Plant lectins as potent Anti-coronaviruses, Anti-inflammatory, antinociceptive and antiulcer agents.Saudi journal of biological sciences · 2022Review
- Purification, characterization and fine sugar specificity of a N-Acetylgalactosamine specific lectin from Adenia hondala.Glycoconjugate journal · 2018Article
- Expression of Lectins in Heterologous Systems.International journal of molecular sciences · 2018Review
- Isolation and Characterization of a Phaseolus vulgaris Trypsin Inhibitor with Antiproliferative Activity on Leukemia and Lymphoma Cells.Molecules (Basel, Switzerland) · 2017Article
- Effect of Nigella sativa alcoholic extract and oil, as well as Phaseolus vulgaris (kidney bean) lectin on the ultrastructure of Trichomonas vaginalis trophozoites.Journal of parasitic diseases : official organ of the Indian Society for Parasitology · 2016Article
- Potential of the Lectin/Inhibitor Isolated from Crataeva tapia Bark (CrataBL) for Controlling Callosobruchus maculatus Larva Development.Journal of agricultural and food chemistry · 2015Article
- A hemagglutinin from northeast red beans with immunomodulatory activity and anti-proliferative and apoptosis-inducing activities toward tumor cells.Protein and peptide letters · 2013Article
- A calcium ion-dependent dimeric bean lectin with antiproliferative activity toward human breast cancer MCF-7 cells.The protein journal · 2013Article
- Molecular modeling of lectin-like protein from Acacia farnesiana reveals a possible anti-inflammatory mechanism in Carrageenan-induced inflammation.BioMed research international · 2013Article
- Lectins: production and practical applications.Applied microbiology and biotechnology · 2011Review
- Extraction and purification of a lectin from red kidney bean and preliminary immune function studies of the lectin and four Chinese herbal polysaccharides.Journal of biomedicine & biotechnology · 2010Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A lectin has been isolated from seeds of the Phaseolus vulgaris cv. "Anasazi beans" using a procedure that involved affinity chromatography on Affi-gel blue gel, fast protein liquid chromatography (FPLC)-ion exchange chromatography on Mono S, and FPLC-gel filtration on Superdex 200. The lectin was comprised of two 30-kDa subunits with substantial N-terminal sequence similarity to other Phaseolus lectins. The hemagglutinating activity of the lectin was stable within the pH range of 1-14 and the temperature range of 0-80 degrees C. The lectin potently suppressed proliferation of MCF-7 (breast cancer) cells with an IC(50) of 1.3 microM, and inhibited the activity of HIV-1 reverse transcriptase with an IC(50) of 7.6 microM. The lectin evoked a mitogenic response from murine splenocytes as evidenced by an increase in [3H-methyl]-thymidine incorporation. The lectin had no antifungal activity. It did not stimulate nitric oxide production by murine peritoneal macrophages. Chemical modification results indicated that tryptophan was crucial for the hemagglutinating activity of the lectin.
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