ArticleAmerican journal of physiology. Gastrointestinal and liver physiology2009
Cross-inhibition between nicotinic acetylcholine receptors and P2X receptors in myenteric neurons and HEK-293 cells.
Article in American journal of physiology. Gastrointestinal and liver physiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 24 citations in OpenAlex.
- Shared mechanisms of dopamine and ATP transmission in the nucleus accumbens.bioRxiv : the preprint server for biology · 2026Article
- Botulinum toxin improved intestinal adaptation to short gut in a twenty-one-day-old weanling rat.Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologica · 2025Article
- Extracellular ATP Neurotransmission and Nicotine Sex-Specifically Modulate Habenular Neuronal Activity in Adolescence.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2023Article
- Circuit-specific enteric glia regulate intestinal motor neurocircuits.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Subtype-specific mechanisms for functional interaction between α6β4* nicotinic acetylcholine receptors and P2X receptors.Molecular pharmacology · 2014Article
- Novel functional roles for enteric glia in the gastrointestinal tract.Nature reviews. Gastroenterology & hepatology · 2012Review
- Mechanisms of constitutive and ATP-evoked ATP release in neonatal mouse olfactory epithelium.BMC neuroscience · 2012Article
- Neuronal P2X2 receptors are mobile ATP sensors that explore the plasma membrane when activated.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2011Article
- Interaction of alpha1D-adrenergic and P2X(7) receptors in the rat lacrimal gland and the effect on intracellular [Ca2+] and protein secretion.Investigative ophthalmology & visual science · 2011Article
- Regulation of GABA(A) receptor dynamics by interaction with purinergic P2X(2) receptors.The Journal of biological chemistry · 2011Article
- GABA(A) Receptors: Post-Synaptic Co-Localization and Cross-Talk with Other Receptors.Frontiers in cellular neuroscience · 2011Article
- Molecular mechanisms of cross-inhibition between nicotinic acetylcholine receptors and P2X receptors in myenteric neurons and HEK-293 cells.Neurogastroenterology and motility · 2010Article
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 2 countries.
Funding
Abstract
The enteric nervous system (ENS) controls gut function. P2X receptors and nicotinic acetylcholine receptors (nAChRs) are ligand-gated cation channels that mediate fast synaptic excitation in the ENS. Close molecular coupling in enteric neuronal membranes contributes to a mutually inhibitory interaction between these receptors; this effect is called cross-inhibition. We studied the molecular mechanisms responsible for cross-inhibition. Whole cell patch-clamp techniques were used to measure P2X- and nAChR-mediated currents in cultured enteric neurons and HEK-293 cells. In cultured myenteric neurons, ACh (3 mM) and ATP (1 mM) coapplication evoked an inward current that was only 57 +/- 6% (P < 0.05) of the predicted current that would have occurred if the two populations of channels were activated independently. In HEK-293 cells coexpressing alpha(3)beta(4) nAChR/P2X(2) receptors, coapplication of ATP and ACh caused a current that was 58 +/- 7% of the predicted current (P < 0.05). To test the importance of P2X subunit COOH-terminal tail length on cross-inhibition, P2X(3) and P2X(4) subunits, which have shorter COOH-terminal tails, were studied. Cross-inhibition with alpha(3)beta(4) nAChRs and P2X(3) or P2X(4) subunits was similar to that occurring with P2X(2) subunits. P2X receptor or alpha(3)beta(4) nAChR desensitization did not prevent receptor cross-inhibition. These data indicate that the alpha(3)beta(4)-P2X receptor interaction is not restricted to P2X(2) subunits. In addition, active and desensitized conformations of the P2X receptor inhibit nAChR function. These molecular interactions may modulate the function of synapses that use ATP and ACh as fast synaptic transmitters in the ENS.
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Registered trials
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