ArticleBMC biotechnology2009
PhiC31 integrase induces a DNA damage response and chromosomal rearrangements in human adult fibroblasts.
Article in BMC biotechnology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
20 citing papers in PubMed, 48 citations in OpenAlex.
- Large serine recombinase-mediated gene insertion for high-throughput screens: advantages, design principles, and applications.Nucleic acids research · 2026Review
- Creation and manipulation of bipartite expression transgenes in C. elegans using phiC31 recombinase.Genetics · 2026Article
- STRAIGHT-IN enables high-throughput targeting of large DNA payloads in human pluripotent stem cells.Cell reports methods · 2022Article
- Global mapping of binding sites for phic31 integrase in transgenic maden-darby bovine kidney cells using ChIP-seq.Hereditas · 2019Article
- A novel λ integrase-mediated seamless vector transgenesis platform for therapeutic protein expression.Nucleic acids research · 2018Article
- Neuroprotective therapies in glaucoma: II. Genetic nanotechnology tools.Frontiers in neuroscience · 2015Review
- Improved site-specific recombinase-based method to produce selectable marker- and vector-backbone-free transgenic cells.Scientific reports · 2014Article
- Hybrid lentivirus-phiC31-int-NLS vector allows site-specific recombination in murine and human cells but induces DNA damage.PloS one · 2014Article
- Less is more: strategies to remove marker genes from transgenic plants.BMC biotechnology · 2013Review
- A site-specific recombinase-based method to produce antibiotic selectable marker free transgenic cattle.PloS one · 2013Article
- Optimization of the piggyBac transposon using mRNA and insulators: toward a more reliable gene delivery system.PloS one · 2013Article
- MiMIC: a highly versatile transposon insertion resource for engineering Drosophila melanogaster genes.Nature methods · 2011Article
- VCre/VloxP and SCre/SloxP: new site-specific recombination systems for genome engineering.Nucleic acids research · 2011Article
- A method for producing transgenic cells using a multi-integrase system on a human artificial chromosome vector.PloS one · 2011Article
- Use of phage φC31 integrase as a tool for zebrafish genome manipulation.Methods in cell biology · 2011Article
- A molecularly defined duplication set for the X chromosome of Drosophila melanogaster.Genetics · 2010Article
- Generation of induced pluripotent stem cells using site-specific integration with phage integrase.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- Biosafety assessment of site-directed transgene integration in human umbilical cord-lining cells.Molecular therapy : the journal of the American Society of Gene Therapy · 2010Article
- Impact of hydrodynamic injection and phiC31 integrase on tumor latency in a mouse model of MYC-induced hepatocellular carcinoma.PloS one · 2010Article
- Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundPhiC31 integrase facilitates efficient integration of transgenes into human and mouse genomes and is considered for clinical gene therapy. However recent studies have shown that the enzyme can induce various chromosomal abnormalities in primary human embryonic cells and mammalian cell lines. The mechanisms involved are unknown, but it has been proposed that PhiC31 attachment sites in the host genome recombine leading to chromosomal translocations.
resultsWe have studied possible effects of the PhiC31 integrase expression in human adult fibroblasts by karyotyping. All control cells were cytogenetically normal, whereas cells expressing PhiC31 integrase show chromosomal abnormalities confirming our previous results using primary embryonic fibroblasts. In order to study the early mechanisms involved we measured H2AX phosphorylation - a primary event in the response to DNA double-strand-breaks. Transient transfection with PhiC31 integrase encoding plasmids lead to an elevated number of cells positive for H2AX phosphorylation detected by immunofluorescence. Western blot analysis confirmed the upregulated H2AX phosphorylation, whereas markers for apoptosis as well as p53 and p21 were not induced. Cells transfected with plasmids encoding the Sleeping Beauty transposase remained cytogenetically normal, and in these cells less upregulation of H2AX phosphorylation could be detected.
conclusionIn primary human fibroblasts expression of PhiC31 integrase leads to a DNA damage response and chromosomal aberrations.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.