ArticleCell proliferation2009
Colonic stem cell data are consistent with the immortal model of stem cell division under non-random strand segregation.
Article in Cell proliferation, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
4 citing papers in PubMed.
- Modeling of stem cell dynamics in human colonic crypts in silico.Journal of gastroenterology · 2014Article
- Sister chromatids segregate at mitosis without mother-daughter bias in Saccharomyces cerevisiae.Genetics · 2012Article
- Tumor-initiating label-retaining cancer cells in human gastrointestinal cancers undergo asymmetric cell division.Stem cells (Dayton, Ohio) · 2012Article
- Colon stem cell and crypt dynamics exposed by cell lineage reconstruction.PLoS genetics · 2011Article
Corrections and comments
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Authors and funding
1 author.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectivesColonic stem cells are thought to reside towards the base of crypts of the colon, but their numbers and proliferation mechanisms are not well characterized. A defining property of stem cells is that they are able to divide asymmetrically, but it is not known whether they always divide asymmetrically (immortal model) or whether there are occasional symmetrical divisions (stochastic model). By measuring diversity of methylation patterns in colon crypt samples, a recent study found evidence in favour of the stochastic model, assuming random segregation of stem cell DNA strands during cell division. Here, the effect of preferential segregation of the template strand is considered to be consistent with the 'immortal strand hypothesis', and explore the effect on conclusions of previously published results. MATERIALS AND
methodsFor a sample of crypts, it is shown how, under the immortal model, to calculate mean and variance of the number of unique methylation patterns allowing for non-random strand segregation and compare them with those observed.
resultsThe calculated mean and variance are consistent with an immortal model that incorporates non-random strand segregation for a range of stem cell numbers and levels of preferential strand segregation.
conclusionsAllowing for preferential strand segregation considerably alters previously published conclusions relating to stem cell numbers and turnover mechanisms. Evidence in favour of the stochastic model may not be as strong as previously thought.
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Registered trials
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