ArticleVirus genes2009
Characterization of Bombyx mori nucleopolyhedrovirus orf74, a novel gene involved in virulence of virus.
Article in Virus genes, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
What it found
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Who cites it
4 citing papers in PubMed.
- Label-free proteomics analysis on the envelope of budded viruses of Bombyx mori nucleopolyhedrovirus harboring differential localized GP64.Virus genes · 2023Article
- Bm65 is essential for the propagation of Bombyx mori nucleopolyhedrovirus.Current microbiology · 2013Article
- Characterization of Bombyx mori nucleopolyhedrovirus with a knockout of Bm17.Cytotechnology · 2012Article
- Use of bacterial artificial chromosomes in baculovirus research and recombinant protein expression: current trends and future perspectives.ISRN microbiology · 2012Article
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
orf74 of Bombyx mori nucleopolyhedrovirus (BmNPV) is a homologue of uncharacterized orf91 of Autographa californica multicapsid NPV, predicted to encode a protein of 17.3 kDa. RT-PCR showed that the transcription of orf74 was initiated at 12 h post-infection stage. Expression assay indicated that ORF74 localized in the nucleus of infected BmN cells. To study the function of ORF74, an orf74-knockout virus was constructed using bacmid technology. Analysis of the production of budded virions and occlusion bodies, the formation of nucleocapsids, and the level of viral DNA revealed no significant difference among the mutant, the control, and the orf74-repaired virus. Interestingly, bioassay showed that the median lethal time of the orf74-knockout virus was 14.7 h longer than the control virus, but the virus yield was similar to that of control virus. Thus, our data indicated that ORF74 is most likely implicated in the virulence of BmNPV, and is not essential for virus replication.
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Registered trials
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