ArticlePloS one2009
Mouse cofactor of BRCA1 (Cobra1) is required for early embryogenesis.
Article in PloS one, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
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Who cites it
45 citing papers in PubMed, 60 citations in OpenAlex.
- Article
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- Deciphering the dialogue between the bovine blastocyst and the uterus: embryo-induced alterations in extracellular vesicle protein content from an ex vivo model and the in vivo environment.Journal of animal science and biotechnology · 2025Article
- Nelfb promotes dermal white adipose tissue formation through RNA polymerase II-mediated adipogenic gene regulation.Development (Cambridge, England) · 2025Article
- Transcriptional elongation control in developmental gene expression, aging, and disease.Molecular cell · 2023Review
- RNA polymerase II pausing temporally coordinates cell cycle progression and erythroid differentiation.Developmental cell · 2023Article
- Dependency of NELF-E-SLUG-KAT2B epigenetic axis in breast cancer carcinogenesis.Nature communications · 2023Article
- RNA Polymerase II pausing temporally coordinates cell cycle progression and erythroid differentiation.medRxiv : the preprint server for health sciences · 2023Article
- RNA Pol II pausing facilitates phased pluripotency transitions by buffering transcription.Genes & development · 2022Article
- Site-specific photolabile roadblocks for the study of transcription elongation in biologically complex systems.Communications biology · 2022Article
- Rapid and efficient degradation of endogenous proteins in vivo identifies stage-specific roles of RNA Pol II pausing in mammalian development.Developmental cell · 2022Article
- RNA polymerase II pausing factor NELF in CD8Nature communications · 2022Article
- Negative Elongation Factor (NELF) Inhibits Premature Granulocytic Development in Zebrafish.International journal of molecular sciences · 2022Article
- Epithelial NELF guards intestinal barrier function to ameliorate colitis by maintaining junctional integrity.Mucosal immunology · 2022Article
- RNA polymerase II pausing in development: orchestrating transcription.Open biology · 2022Review
- Negative elongation factor regulates muscle progenitor expansion for efficient myofiber repair and stem cell pool repopulation.Developmental cell · 2021Article
- Negative elongation factor complex enables macrophage inflammatory responses by controlling anti-inflammatory gene expression.Nature communications · 2020Article
- NELF Regulates a Promoter-Proximal Step Distinct from RNA Pol II Pause-Release.Molecular cell · 2020Article
- Promoter-proximal pausing of RNA polymerase II: a nexus of gene regulation.Genes & development · 2019Review
- Negative elongation factor is essential for endometrial function.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2019Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundNegative elongation factor (NELF) is a four-subunit protein complex conserved from Drosophila to humans. In vitro biochemical and tissue culture-based studies have demonstrated an important role of NELF in controlling RNA polymerase II (Pol II) pausing in transcription. However, the physiological significance of NELF function is not clear due to the lack of any genetic systems for studying NELF. PRINCIPAL
findingsHere we show that disruption of the mouse B subunit of NELF (NELF-B), also known as cofactor of BRCA1 (Cobra1), causes inner cell mass (ICM) deficiency and embryonic lethality at the time of implantation. Consistent with the phenotype of the Cobra1 knockout (KO) embryos, knockdown of Cobra1 in mouse embryonic stem cells (ESCs) reduces the efficiency of colony formation and increases spontaneous differentiation. Cobra1-depleted ESCs maintain normal levels of Oct4, Nanog, and Sox2, master regulators of pluripotency in ESCs. However, knockdown of Cobra1 leads to precocious expression of developmental regulators including lymphoid enhancer-binding factor 1 (Lef1). Chromatin immunoprecipitation (ChIP) indicates that Cobra1 binds to the Lef1 promoter and modulates the abundance of promoter-bound RNA polymerase.
conclusionsCobra1 is essential for early embryogenesis. Our findings also indicate that Cobra1 helps maintain the undifferentiated state of mESCs by preventing unscheduled expression of developmental genes.
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Registered trials
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