ArticlePloS one2009
Inducible transgenic rat model for diabetes mellitus based on shRNA-mediated gene knockdown.
Article in PloS one, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 39 citations in OpenAlex.
- Poor Decision Making and Sociability Impairment Following Central Serotonin Reduction in Inducible TPH2-Knockdown Rats.International journal of molecular sciences · 2024Article
- Diabetic pregnancy as a novel risk factor for cardiac dysfunction in the offspring-the heart as a target for fetal programming in rats.Diabetologia · 2021Article
- Impact of maternal hyperglycemia on cardiac development: Insights from animal models.Genesis (New York, N.Y. : 2000) · 2021Review
- Effects of empagliflozin and target-organ damage in a novel rodent model of heart failure induced by combined hypertension and diabetes.Scientific reports · 2020Article
- Accurate assessment of LV function using the first automated 2D-border detection algorithm for small animals - evaluation and application to models of LV dysfunction.Cardiovascular ultrasound · 2019Article
- Continuous Blood Glucose Monitoring Reveals Enormous Circadian Variations in Pregnant Diabetic Rats.Frontiers in endocrinology · 2018Article
- Intelectin contributes to allergen-induced IL-25, IL-33, and TSLP expression and type 2 response in asthma and atopic dermatitis.Mucosal immunology · 2017Article
- The TetO rat as a new translational model for type 2 diabetic retinopathy by inducible insulin receptor knockdown.Diabetologia · 2017Article
- Oral administration of angiotensin-(1-7) ameliorates type 2 diabetes in rats.Journal of molecular medicine (Berlin, Germany) · 2014Article
- Synthetic microRNA-mediated downregulation of Nogo-A in transgenic rats reveals its role as regulator of synaptic plasticity and cognitive function.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- On the emerging role of rabbit as human disease model and the instrumental role of novel transgenic tools.Transgenic research · 2012Review
- In vivo optical imaging and dynamic contrast methods for biomedical research.Philosophical transactions. Series A, Mathematical, physical, and engineering sciences · 2011Review
- RNA interference in pigs: comparison of RNAi test systems and expression vectors.Molecular biotechnology · 2011Article
- Derivation, characterization, and stable transfection of induced pluripotent stem cells from Fischer344 rats.PloS one · 2011Article
- Generation and characterization of a Tet-On (rtTA-M2) transgenic rat.BMC developmental biology · 2010Article
- Sequence-non-specific effects of RNA interference triggers and microRNA regulators.Nucleic acids research · 2010Review
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The rat is an important animal model in biomedical research, but gene targeting technology is not established for this species. Therefore, we aimed to produce transgenic knockdown rats using shRNA technology and pronuclear microinjection. To this purpose, we employed a tetracycline-inducible shRNA expression system targeting the insulin receptor (IR). Doxycycline (DOX) treatment of the resulting transgenic rats led to a dose-dependent and reversible increase in blood glucose caused by ubiquitous inhibition of IR expression and signalling. We could neither detect an interferon response nor disturbances in microRNA processing after DOX treatment excluding toxic effects of shRNA expression. Low dose DOX treatment induced a chronic state of diabetes mellitus. In conclusion, we have developed a technology which allows the specific, inducible, and reversible suppression of any gene of interest in the rat. Our first transgenic rat line generated with this method represents an inducible model for diabetes mellitus.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.