ArticleBiology of reproduction2009
Manipulating actin dynamics affects human in vitro decidualization.
Article in Biology of reproduction, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
23 citing papers in PubMed, 49 citations in OpenAlex.
- Progesterone signaling in cardio-renal physiology and pathophysiology.Frontiers in endocrinology · 2026Review
- Loss of Parkinson Disease Protein 7 (PARK7) upregulates ROS and cell migration and is associated with recurrent pregnancy loss.Molecular medicine (Cambridge, Mass.) · 2025Article
- Primary cilia prevent activation of the cGAS-STING pathway during mouse decidualization.Communications biology · 2025Article
- Nuclear actin assembly is an integral part of decidualization in human endometrial stromal cells.Communications biology · 2024Article
- ATOH8 Expression Is Regulated by BMP2 and Plays a Key Role in Human Endometrial Stromal Cell Decidualization.Endocrinology · 2023Article
- β-Lactoglobulin affects the oxidative status and viability of equine endometrial progenitor cells via lncRNA-mRNA-miRNA regulatory associations.Journal of cellular and molecular medicine · 2023Article
- Immune Tolerance of Embryo Implantation and Pregnancy: The Role of Human Decidual Stromal Cell- and Embryonic-Derived Extracellular Vesicles.International journal of molecular sciences · 2022Review
- TOP2A deficit-induced abnormal decidualization leads to recurrent implantation failure via the NF-κB signaling pathway.Reproductive biology and endocrinology : RB&E · 2022Article
- Three-dimensional microengineered vascularised endometrium-on-a-chip.Human reproduction (Oxford, England) · 2021Article
- Immune Tolerance of the Human Decidua.Journal of clinical medicine · 2021Review
- Flow-enhanced priming of hESCs through H2B acetylation and chromatin decondensation.Stem cell research & therapy · 2019Article
- Defective decidualization during and after severe preeclampsia reveals a possible maternal contribution to the etiology.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- miR-181b-5p Modulates Cell Migratory Proteins, Tissue Inhibitor of Metalloproteinase 3, and Annexin A2 During In Vitro Decidualization in a Human Endometrial Stromal Cell Line.Reproductive sciences (Thousand Oaks, Calif.) · 2017Article
- Characterization and predicted role of the microRNA expression profile in amnion from obese pregnant women.International journal of obesity (2005) · 2014Article
- High cofilin-1 levels correlate with cisplatin resistance in lung adenocarcinomas.Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine · 2014Article
- Interdisciplinary collaborative team for blastocyst implantation research: inception and perspectives.American journal of reproductive immunology (New York, N.Y. : 1989) · 2014Article
- Comparative proteomic analysis of kidney development-related proteins in the pig.In vitro cellular & developmental biology. Animal · 2013Article
- Interaction of the conceptus and endometrium to establish pregnancy in mammals: role of interleukin 1β.Cell and tissue research · 2012Review
- Kruppel-like factor 5 (KLF5) is critical for conferring uterine receptivity to implantation.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- miRNA signature and Dicer requirement during human endometrial stromal decidualization in vitro.PloS one · 2012Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
The differentiation of uterine stromal fibroblasts into decidual cells is critical for establishing pregnancy. This process, called decidualization, requires the reorganization of the actin cytoskeleton, which mainly depends on actin dynamics and the phosphorylation status of the myosin light chain. We manipulated actin dynamics with jasplakinolide (100 nM) and latrunculin B (1 microM), both of which significantly inhibited the synthesis of decidualization markers induced by 6 days of treatment with embryo-mimicking stimulus interleukin 1beta (IL1B) and steroid hormones (SHs; 17beta-estradiol and medroxyprogesterone acetate) in the human uterine fibroblast (HuF) in vitro model. However, only jasplakinolide had long-lasting effects on the G-actin:F-actin ratio and prevented decidualization induced by the artificial stimulus cAMP (and SHs). Actin-binding protein cofilin mainly colocalized with G-actin in the nucleus as well as the cytoplasm. Only some spots of colocalization between cofilin and F-actin were detected in the cytoplasm. Brief extraction of cytosolic proteins from living cells revealed that in cells treated with IL1B or cAMP (and SHs) for 6 days, cofilin was mainly detected in the nucleus. The translocation of cofilin from cytosol to nucleus was also detected in HuFs treated for 12 days with SHs, IL1B and SHs, and cAMP and SHs. The same significant translocation was confirmed in primary baboon stromal uterine fibroblasts. We conclude that changes in actin dynamics, particularly the stabilization of F-actin, have a significant negative impact on decidualization, and the translocation of cofilin to the nucleus is a key feature of this process in the primate.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.