ArticleJournal of clinical microbiology2009
New molecular detection tools adapted to emerging rhinoviruses and enteroviruses.
Article in Journal of clinical microbiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 55 papers, 1 of them a synthesis that pooled it.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
55 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Practical Guidance for Clinical Microbiology Laboratories: Viruses Causing Acute Respiratory Tract Infections.Clinical microbiology reviews · 2019Guideline
- Virus-triggered exacerbation in allergic asthmatic children: neutrophilic airway inflammation and alteration of virus sensors characterize a subgroup of patients.Respiratory research · 2017Trial
- Efficacy and safety of iota-carrageenan nasal spray versus placebo in early treatment of the common cold in adults: the ICICC trial.Respiratory research · 2015Trial
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- Aetiology of acute respiratory infection in Vientiane, Lao PDR, from a case-control study.Scientific reports · 2026Article
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- Seasonality and Co-Detection of Respiratory Viral Infections Among Hospitalised Patients Admitted With Acute Respiratory Illness-Valencia Region, Spain, 2010-2021.Influenza and other respiratory viruses · 2024Article
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- Etiologies of Acute Bronchiolitis in Children at Risk for Asthma, with Emphasis on the Human Rhinovirus Genotyping Protocol.Journal of clinical medicine · 2023Article
- Frequent detection and genotyping of human rhinovirus in SARS-CoV-2 negative patients; a study from south of Iran.Iranian journal of microbiology · 2023Article
- Identification of a Conserved, Linear Epitope on VP3 ofViruses · 2023Article
- Longitudinal Detection of Twenty DNA and RNA Viruses in Allogeneic Hematopoietic Stem Cell Transplant Recipients Plasma.Viruses · 2023Observational
- Comparison of tissue tropism and host response to enteric and respiratory enteroviruses.PLoS pathogens · 2022Article
- Trends and Intensity of Rhinovirus Invasions in Kilifi, Coastal Kenya, Over a 12-Year Period, 2007-2018.Open forum infectious diseases · 2021Article
- Development of Novel PCR Assays for Improved Detection of Enterovirus D68.Journal of clinical microbiology · 2021Article
- Detection ofJournal of fungi (Basel, Switzerland) · 2021Article
- Microscopic Observation of SARS-Like Particles in RT-qPCR SARS-CoV-2 Positive Sewage Samples.Pathogens (Basel, Switzerland) · 2021Article
- Detection of Parechovirus A1 with Monoclonal Antibody against Capsid Protein VP0.Microorganisms · 2020Article
- Enteroviruses from Humans and Great Apes in the Republic of Congo: Recombination within Enterovirus C Serotypes.Microorganisms · 2020Article
- Point-of-care multiplexed diagnosis of meningitis using the FilmArray® ME panel technology.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2020Article
Corrections and comments
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Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Human rhinoviruses (HRV), and to a lesser extent human enteroviruses (HEV), are important respiratory pathogens. Like other RNA viruses, these picornaviruses have an intrinsic propensity to variability. This results in a large number of different serotypes as well as the incessant discovery of new genotypes. This large and growing diversity not only complicates the design of real-time PCR assays but also renders immunofluorescence unfeasible for broad HRV and HEV detection or quantification in cells. In this study, we used the 5' untranslated region, the most conserved part of the genome, as a target for the development of both a real-time PCR assay (Panenterhino/Ge/08) and a peptide nucleic acid-based hybridization oligoprobe (Panenterhino/Ge/08 PNA probe) designed to detect all HRV and HEV species members according to publicly available sequences. The reverse transcription-PCR assay has been validated, using not only plasmid and viral stocks but also quantified RNA transcripts and around 1,000 clinical specimens. These new generic detection PCR assays overcame the variability of circulating strains and lowered the risk of missing emerging and divergent HRV and HEV. An additional real-time PCR assay (Entero/Ge/08) was also designed specifically to provide sensitive and targeted detection of HEV in cerebrospinal fluid. In addition to the generic probe, we developed specific probes for the detection of HRV-A and HRV-B in cells. This investigation provides a comprehensive toolbox for accurate molecular identification of the different HEV and HRV circulating in humans.
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