ArticleBiotechnology and bioengineering2009
A novel high-throughput cell-based method for integrated quantification of type I interferons and in vitro screening of immunostimulatory RNA drug delivery.
Article in Biotechnology and bioengineering, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
11 citing papers in PubMed, 18 citations in OpenAlex.
- High-throughput screening of nanoparticles in drug delivery.APL bioengineering · 2021Review
- Cigarette smoke dampens antiviral signaling in small airway epithelial cells by disrupting TLR3 cleavage.American journal of physiology. Lung cellular and molecular physiology · 2018Article
- High-throughput approaches for screening and analysis of cell behaviors.Biomaterials · 2018Review
- Influenza A virus attenuation by codon deoptimization of the NS gene for vaccine development.Journal of virology · 2014Article
- Visualizing infection spread: dual-color fluorescent reporting of virus-host interactions.Biotechnology and bioengineering · 2014Article
- A Sendai virus-derived RNA agonist of RIG-I as a virus vaccine adjuvant.Journal of virology · 2013Article
- Article
- Lipid-derived nanoparticles for immunostimulatory RNA adjuvant delivery.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Identification of small molecules with type I interferon inducing properties by high-throughput screening.PloS one · 2012Article
- Article
- Evaluating the orthopoxvirus type I interferon-binding molecule as a vaccine target in the vaccinia virus intranasal murine challenge model.Clinical and vaccine immunology : CVI · 2010Article
Corrections and comments
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Authors and funding
7 authors at 5 institutions in 1 country.
Funding
Abstract
A hallmark of immune activation by certain RNA sequences is the generation of interferon responses. However, the study of immunostimulatory RNA (isRNA) has been hindered by costly and slow methods, particularly in vitro. We have developed a cell-based assay to detect human type I interferon (IFN) that reliably senses both IFN-alpha and IFN-beta simultaneously. The human 293T cell line was stably transfected with a fusion gene of monomeric red fluorescent protein (mRFP) under the transcriptional control of an interferon-stimulated response element (ISRE). High levels of mRFP are expressed following activation of the type I IFN receptor (IFNAR). Using this method, detection limits for IFN similar to that of ELISA can be achieved but with a greater dynamic range and in a high-throughput manner. As a proof of concept, we utilized this method to screen a library of cationic lipid-like materials that form nanoparticle complexes with RNA for induction of innate immune responses in vitro. We expect the screening and detection methods described herein may provide a useful tool in elucidating mechanisms that govern the delivery of RNA molecules to effector cells and receptors of the innate immune system. We apply this tool to investigate isRNA drug delivery, but it may also find use in other applications for which high-throughput detection of type 1 IFN is desired.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.