Evidence map›Paper›PMID 19337736›Full record

ArticlePlant cell reports2009

Expression of the cry1EC gene in castor (Ricinus communis L.) confers field resistance to tobacco caterpillar (Spodoptera litura Fabr) and castor semilooper (Achoea janata L.).

M Sujatha, M Lakshminarayana, M Tarakeswari, P K Singh, Rakesh Tuli

Abstract read
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In one paragraph

Article in Plant cell reports, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

M SujathaDirectorate of Oilseeds Research, Rajendranagar, Hyderabad, 500 030, India. mulpurisujata@yahoo.com
M Lakshminarayana
M Tarakeswari
P K Singh
Rakesh Tuli

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Castor (cv. DCS-9) has been transformed through Agrobacterium-mediated and particle gun bombardment methods using appropriate vectors containing the Bt chimeric gene cry1EC driven by enhanced 35S promoter. About 81 and 12 putative transformants were regenerated following selection on hygromycin and kanamycin, respectively. Southern analysis of DNA extracted from T(0) plants confirmed integration of the introduced gene in castor genome. The integration and inheritance of the introduced genes was demonstrated up to T(4) generation by PCR and Southern analysis. Southern analysis of two events having single and two copies showed the same pattern of integration in the subsequent generations. Insect feeding experiments conducted in the laboratory by releasing neonate larvae of castor semilooper and S. litura on leaf tissues excised from transgenic and control plants showed varying degrees of larval mortality and slow growth in larvae fed on transgenic leaf tissue. Field bioassays against Spodoptera litura and castor semilooper conducted for eight events in T(1)-T(4) generations under net confinement were more informative and events conferring resistance to the two major defoliators were identified.

Indexed as

MothsSpodopteraAnimalsBacillus thuringiensis ToxinsBacterial ProteinsDNA, PlantEndotoxinsGene Expression Regulation, BacterialGenetic VectorsGene Transfer TechniquesHemolysin ProteinsLarvaPlants, Genetically ModifiedRhizobiumRicinus communisTransformation, GeneticBacillus thuringiensis ToxinsBacterial ProteinsDNA, PlantEndotoxinsHemolysin Proteinsinsecticidal crystal protein, Bacillus Thuringiensis

Identifiers

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.