ArticlePloS one2009
Paracrine diffusion of PrP(C) and propagation of prion infectivity by plasma membrane-derived microvesicles.
Article in PloS one, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
22 citing papers in PubMed, 46 citations in OpenAlex.
- Efficient enzyme-free isolation of brain-derived extracellular vesicles.Journal of extracellular vesicles · 2024Article
- Propagation and Dissemination Strategies of Transmissible Spongiform Encephalopathy Agents in Mammalian Cells.International journal of molecular sciences · 2022Review
- Highly efficient intercellular spreading of protein misfolding mediated by viral ligand-receptor interactions.Nature communications · 2021Article
- Cerebrospinal Fluid and Plasma Small Extracellular Vesicles and miRNAs as Biomarkers for Prion Diseases.International journal of molecular sciences · 2021Article
- Extracellular microvesicles/exosomes: discovery, disbelief, acceptance, and the future?Leukemia · 2020Review
- Cellular mechanisms responsible for cell-to-cell spreading of prions.Cellular and molecular life sciences : CMLS · 2018Review
- Peptidylarginine Deiminases-Roles in Cancer and Neurodegeneration and Possible Avenues for Therapeutic Intervention via Modulation of Exosome and Microvesicle (EMV) Release?International journal of molecular sciences · 2017Review
- Review
- Efficient inhibition of infectious prions multiplication and release by targeting the exosomal pathway.Cellular and molecular life sciences : CMLS · 2015Article
- Extracellular vesicles in Alzheimer's disease: friends or foes? Focus on aβ-vesicle interaction.International journal of molecular sciences · 2015Review
- Prion strains are differentially released through the exosomal pathway.Cellular and molecular life sciences : CMLS · 2015Article
- The neutral sphingomyelinase pathway regulates packaging of the prion protein into exosomes.The Journal of biological chemistry · 2015Article
- Extracellular vesicles from neural stem cells transfer IFN-γ via Ifngr1 to activate Stat1 signaling in target cells.Molecular cell · 2014Article
- First demonstration of transmissible spongiform encephalopathy-associated prion protein (PrPTSE) in extracellular vesicles from plasma of mice infected with mouse-adapted variant Creutzfeldt-Jakob disease by in vitro amplification.The Journal of biological chemistry · 2014Article
- Microglia convert aggregated amyloid-β into neurotoxic forms through the shedding of microvesicles.Cell death and differentiation · 2014Article
- Plasma membrane invaginations containing clusters of full-length PrPSc are an early form of prion-associated neuropathology in vivo.Neurobiology of aging · 2013Article
- Recruitment of cellular prion protein to mitochondrial raft-like microdomains contributes to apoptosis execution.Molecular biology of the cell · 2011Article
- Misfolded protein aggregates: mechanisms, structures and potential for disease transmission.Seminars in cell & developmental biology · 2011Review
- All clinically-relevant blood components transmit prion disease following a single blood transfusion: a sheep model of vCJD.PloS one · 2011Article
- Function of membrane rafts in viral lifecycles and host cellular response.Biochemistry research international · 2011Article
Corrections and comments
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Authors and funding
10 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cellular prion protein (PrP(C)) is a physiological constituent of eukaryotic cells. The cellular pathways underlying prions spread from the sites of prions infection/peripheral replication to the central nervous system are still not elucidated. Membrane-derived microvesicles (MVs) are submicron (0.1-1 microm) particles, that are released by cells during plasma membrane shedding processes. They are usually liberated from different cell types, mainly upon activation as well as apoptosis, in this case, one of their hallmarks is the exposure of phosphatidylserine in the outer leaflet of the membrane. MVs are also characterized by the presence of adhesion molecules, MHC I molecules, as well as of membrane antigens typical of their cell of origin. Evidence exists that MVs shedding provide vehicles to transfer molecules among cells, and that MVs are important modulators of cell-to-cell communication. In this study we therefore analyzed the potential role of membrane-derived MVs in the mechanism(s) of PrP(C) diffusion and prion infectivity transmission. We first identified PrP(C) in association with the lipid raft components Fyn, flotillin-2, GM1 and GM3 in MVs from plasma of healthy human donors. Similar findings were found in MVs from cell culture supernatants of murine neuronal cells. Furthermore we demonstrated that PrP(Sc) is released from infected murine neuronal cells in association with plasma membrane-derived MVs and that PrP(Sc)-bearing MVs are infectious both in vitro and in vivo. The data suggest that MVs may contribute both to the intercellular mechanism(s) of PrP(C) diffusion and signaling as well as to the process of prion spread and neuroinvasion.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.