ArticleNucleic acids research2009
Cotranscriptional recruitment of the nuclear poly(A)-binding protein Pab2 to nascent transcripts and association with translating mRNPs.
Article in Nucleic acids research, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 32 citations in OpenAlex.
- Fission yeast poly(A) polymerase active site mutation Y86D alleviates theRNA (New York, N.Y.) · 2023Article
- PABPN1 prevents the nuclear export of an unspliced RNA with a constitutive transport element and controls human gene expression via intron retention.RNA (New York, N.Y.) · 2023Article
- PABPN1, a Target of p63, Modulates Keratinocyte Differentiation through Regulation of p63α mRNA Translation.The Journal of investigative dermatology · 2020Article
- LSM2-8 and XRN-2 contribute to the silencing of H3K27me3-marked genes through targeted RNA decay.Nature cell biology · 2020Article
- A Conserved Mito-Cytosolic Translational Balance Links Two Longevity Pathways.Cell metabolism · 2020Article
- Common mechanism of transcription termination at coding and noncoding RNA genes in fission yeast.Nature communications · 2018Article
- The nuclear poly(A) binding protein of mammals, but not of fission yeast, participates in mRNA polyadenylation.RNA (New York, N.Y.) · 2017Article
- Nuclear poly(A)-binding protein aggregates misplace a pre-mRNA outside of SC35 speckle causing its abnormal splicing.Nucleic acids research · 2016Article
- DDX6 Orchestrates Mammalian Progenitor Function through the mRNA Degradation and Translation Pathways.Molecular cell · 2015Article
- RNA processing factors Swd2.2 and Sen1 antagonize RNA Pol III-dependent transcription and the localization of condensin at Pol III genes.PLoS genetics · 2014Article
- PABPN1: molecular function and muscle disease.The FEBS journal · 2013Review
- Introns regulate gene expression in Cryptococcus neoformans in a Pab2p dependent pathway.PLoS genetics · 2013Article
- The THO complex cooperates with the nuclear RNA surveillance machinery to control small nucleolar RNA expression.Nucleic acids research · 2012Article
- Article
- Widespread cotranslational formation of protein complexes.PLoS genetics · 2011Article
- Red1 promotes the elimination of meiosis-specific mRNAs in vegetatively growing fission yeast.The EMBO journal · 2011Article
- MOS11: a new component in the mRNA export pathway.PLoS genetics · 2010Article
- Negative regulation of meiotic gene expression by the nuclear poly(a)-binding protein in fission yeast.The Journal of biological chemistry · 2010Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Synthesis of the pre-mRNA poly(A) tail in the nucleus has important consequences on the translational activity of the mature mRNA in the cytoplasm. In most eukaryotes, nuclear polyadenylation of pre-mRNAs is thought to require the nuclear poly(A)-binding protein (PABP2/PABPN1) for poly(A) tail synthesis and ultimate length control. As yet, however, the extent of the association between PABP2 and the exported mRNA remains poorly understood. Here, we used chromatin immunoprecipitation (ChIP) assays to show that the fission yeast ortholog of mammalian PABP2 (Pab2) is cotranscriptionally recruited to active genes. Notably, the association of Pab2 to genes precedes that of a typical 3'-processing/polyadenylation factor, suggesting that Pab2 recruitment during the transcription cycle precedes polyadenylation. The inclusion of an RNase step in our ChIP and immunoprecipitation assays suggests that Pab2 is cotranscriptionally recruited via nascent mRNA ribonucleoprotein (mRNPs). Tandem affinity purification coupled with mass spectrometry also revealed that Pab2 associates with several ribosomal proteins as well as general translation factors. Importantly, whereas previous results suggest that the nuclear poly(A)-binding protein is not present on cytoplasmic mRNAs, we show that fission yeast Pab2 is associated with polysomes. Our findings suggest that Pab2 is recruited to nascent mRNPs during transcription and remains associated with translated mRNPs after nuclear export.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.