ArticleNucleic acids research2009
Linker histone H1 is present in centromeric chromatin of living human cells next to inner kinetochore proteins.
Article in Nucleic acids research, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 18 citations in OpenAlex.
- Linker histone H1.5 contributes to centromere integrity.Nucleic acids research · 2026Article
- CENP-N promotes the compaction of centromeric chromatin.Nature structural & molecular biology · 2022Article
- Mechanical properties of nucleoprotein complexes determined by nanoindentation spectroscopy.Nucleus (Austin, Tex.) · 2020Article
- The NF2 tumor suppressor merlin interacts with Ras and RasGAP, which may modulate Ras signaling.Oncogene · 2019Article
- Genome distribution of replication-independent histone H1 variants shows H1.0 associated with nucleolar domains and H1X associated with RNA polymerase II-enriched regions.The Journal of biological chemistry · 2015Article
- The CENP-T C-terminus is exclusively proximal to H3.1 and not to H3.2 or H3.3.International journal of molecular sciences · 2015Article
- Mapping of six somatic linker histone H1 variants in human breast cancer cells uncovers specific features of H1.2.Nucleic acids research · 2014Article
- A CENP-S/X complex assembles at the centromere in S and G2 phases of the human cell cycle.Open biology · 2014Article
- Step-wise assembly, maturation and dynamic behavior of the human CENP-P/O/R/Q/U kinetochore sub-complex.PloS one · 2012Article
- Dynamics of CENP-N kinetochore binding during the cell cycle.Journal of cell science · 2011Article
- Down the rabbit hole of centromere assembly and dynamics.Current opinion in cell biology · 2010Review
- Acceptor-photobleaching FRET analysis of core kinetochore and NAC proteins in living human cells.European biophysics journal : EBJ · 2009Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The vertebrate kinetochore complex assembles at the centromere on alpha-satellite DNA. In humans, alpha-satellite DNA has a repeat length of 171 bp slightly longer than the DNA in the chromatosome containing the linker histone H1. The centromere-binding protein CENP-B binds specifically to alpha-satellite DNA with properties of a centromeric-linker histone. Here, we analysed if linker histone H1 is present at or excluded from centromeric chromatin by CENP-B. By immunostaining we detected the presence, but no enrichment or depletion of five different H1 subtypes at centromeric chromatin. The binding dynamics of H1 at centromeric sites were similar to that at other locations in the genome. These dynamics did not change in CENP-B depleted cells, suggesting that CENP-B and H1 co-exist in centromeric chromatin with no or little functional overlap. By bimolecular fluorescence complementation (BiFC) and Förster resonance energy transfer (FRET), we revealed that the linker histone H1 subtypes H1 degrees and H1.2 bind to centromeric chromatin in interphase nuclei in direct neighbourhood to inner kinetochore proteins.
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