ArticleNucleic acids research2009
Rapid antibody selection by mRNA display on a microfluidic chip.
Article in Nucleic acids research, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Hepatitis B virus evades the immune system by suppressing the NF-κB signaling pathway with DENND2A.Microbiology spectrum · 2024Article
- Progresses in Cell-Free In Vitro Evolution.Advances in biochemical engineering/biotechnology · 2023Review
- Directing evolution of novel ligands by mRNA display.Chemical Society reviews · 2021Review
- In Pursuit of Zero 2.0: Recent Developments in Nonfouling Polymer Brushes for Immunoassays.Advanced materials (Deerfield Beach, Fla.) · 2020Review
- Enhanced mRNA-protein fusion efficiency of a single-domain antibody by selection of mRNA display with additional random sequences in the terminal translated regions.Biophysics and physicobiology · 2017Article
- Endosomal escape efficiency of fusogenic B18 and B55 peptides fused with anti-EGFR single chain Fv as estimated by nuclear translocation.Journal of biochemistry · 2016Article
- Overview of protein microarrays.Current protocols in protein science · 2013Review
- In vitro selection of proteins with desired characteristics using mRNA-display.Methods (San Diego, Calif.) · 2013Article
- MIP-2A is a novel target of an anilinoquinazoline derivative for inhibition of tumour cell proliferation.PloS one · 2013Article
- Directed evolution of lectins with sugar-binding specificity for 6-sulfo-galactose.The Journal of biological chemistry · 2012Article
- An anilinoquinazoline derivative inhibits tumor growth through interaction with hCAP-G2, a subunit of condensin II.PloS one · 2012Article
- In vitro selection of fab fragments by mRNA display and gene-linking emulsion PCR.Journal of nucleic acids · 2012Article
- A phthalimide derivative that inhibits centrosomal clustering is effective on multiple myeloma.PloS one · 2012Article
- mRNA display using covalent coupling of mRNA to translated proteins.Methods in molecular biology (Clifton, N.J.) · 2012Article
- Advantages of mRNA display selections over other selection techniques for investigation of protein-protein interactions.Expert review of proteomics · 2011Review
- Bicistronic DNA display for in vitro selection of Fab fragments.Nucleic acids research · 2009Article
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In vitro antibody-display technologies are powerful approaches for isolating monoclonal antibodies from recombinant antibody libraries. However, these display techniques require several rounds of affinity selection which is time-consuming. Here, we combined mRNA display with a microfluidic system for in vitro selection and evolution of antibodies and achieved ultrahigh enrichment efficiency of 10(6)- to 10(8)-fold per round. After only one or two rounds of selection, antibodies with high affinity and specificity were obtained from naive and randomized single-chain Fv libraries of approximately 10(12) molecules. Furthermore, we confirmed that not only protein-protein (antigen-antibody) interactions, but also protein-DNA and protein-drug interactions were selected with ultrahigh efficiencies. This method will facilitate high-throughput preparation of antibodies and identification of protein interactions in proteomic and therapeutic fields.
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Registered trials
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