ArticleCell biology and toxicology1991
HgCl2-induced alteration of actin filaments in cultured primary rat proximal tubule epithelial cells labelled with fluorescein phalloidin.
Article in Cell biology and toxicology, 1991. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 45 citations in OpenAlex.
- Oncosis-inducing cyclometalated iridium(iii) complexes.Chemical science · 2018Article
- High-throughput imaging-based nephrotoxicity prediction for xenobiotics with diverse chemical structures.Archives of toxicology · 2016Article
- Article
- Disruption of calcium homeostasis in cardiomyocytes underlies cardiac structural and functional changes in severe sepsis.PloS one · 2013Article
- Calpain-mediated breakdown of cytoskeletal proteins contributes to cholecystokinin-induced damage of rat pancreatic acini.International journal of experimental pathology · 2009Article
- Dynamic effects of Hg2+-induced changes in cell volume.Cell biochemistry and biophysics · 2008Article
- Gold Nanorods Mediate Tumor Cell Death by Compromising Membrane Integrity.Advanced materials (Deerfield Beach, Fla.) · 2007Article
- Studies on the mechanisms and kinetics of apoptosis induced by microinjection of cytochrome c in rat kidney tubule epithelial cells (NRK-52E).The American journal of pathology · 2000Article
- Acoustic overstimulation increases outer hair cell Ca2+ concentrations and causes dynamic contractions of the hearing organ.Proceedings of the National Academy of Sciences of the United States of America · 1998Article
- Pig kidney (LLC-PK1) cell membrane fluidity during exposure to gentamicin or tobramycin.Antimicrobial agents and chemotherapy · 1994Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
When proximal tubule epithelial cells are exposed to HgCl2, cytoplasmic blebs are formed. These represent an early, potentially reversible response to injury. These blebs are accompanied by reorganization of cytoskeletal proteins, and presumably by alternations in cytoskeletal-plasma membrane interactions. Ca(2+)-activated proteinases, such as calpain, are known to affect cytoskeletal proteins and to be involved in diverse cellular processes. However, the role of calpains in cytotoxicity due to HgCl2 is unknown. To determine the relationship between F-actin, calpain, and HgCl2 toxicity, cells were stained with fluorescein phalloidin before and after treatment with HgCl2. Cells were grown on coverslips and exposed to HgCl2 (10 or 25 microM) in the presence or absence of the calpain inhibitor, leupeptin. Untreated cells were flat, polygonal, and contained many fluorescent-stained cables of actin filaments. Generally, cells exposed to HgCl2 became pleomorphic and contracted as the blebs formed. These cells showed fewer actin cables and fluorescence was seen mostly as either compact areas of dense stain or as peripheral rings. In many cells, actin cables and filaments were completely absent. Disappearance of F-actin was initially seen by 2 min after exposure to HgCl2. Thus, disruption of the actin cytoskeleton and blebbing were found to be early events in HgCl2 toxicity. When leupeptin was used with HgCl2 treatment, the actin staining appeared similar to that of untreated cells.
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