ArticlePloS one2009
A high-speed congenic strategy using first-wave male germ cells.
Article in PloS one, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
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- MUC16-dependent Renal Vascular Adhesion of Candida Promotes Tissue Invasion and Predicts Clinical Outcome in Candidemia.Research square · 2026Article
- Generation of functionally competent testicular somatic cells from pluripotent stem cells.Science advances · 2026Article
- Morc1 reestablishes H3K9me3 heterochromatin on piRNA-targeted transposons in gonocytes.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- A timely, user-friendly, and flexible marker-assisted speed congenics method.Transgenic research · 2023Article
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- Development of assisted reproductive technologies in small animal species for their efficient preservation and production.The Journal of reproduction and development · 2020Review
- Early production of offspring by in vitro fertilization using first-wave spermatozoa from prepubertal male mice.The Journal of reproduction and development · 2019Article
- Comparative study of environmental pollutants bisphenol A and bisphenol S on sexual differentiation of anteroventral periventricular nucleus and spermatogenesis.Reproductive biology and endocrinology : RB&E · 2019Article
- Article
- Development of reproductive engineering techniques at the RIKEN BioResource Center.Experimental animals · 2017Review
- High-Speed Mouse Backcrossing Through the Female Germ Line.PloS one · 2016Article
- CTRP6 is an endogenous complement regulator that can effectively treat induced arthritis.Nature communications · 2015Article
- Simple gamete preservation and artificial reproduction of mammals using micro-insemination techniques.Reproductive medicine and biology · 2015Review
- Validation of simple sequence length polymorphism regions of commonly used mouse strains for marker assisted speed congenics screening.International journal of genomics · 2015Article
- Large, male germ cell-specific hypomethylated DNA domains with unique genomic and epigenomic features on the mouse X chromosome.DNA research : an international journal for rapid publication of reports on genes and genomes · 2013Article
- t-SNARE Syntaxin2 (STX2) is implicated in intracellular transport of sulfoglycolipids during meiotic prophase in mouse spermatogenesis.Biology of reproduction · 2013Article
- Neutrophil infiltration during inflammation is regulated by PILRα via modulation of integrin activation.Nature immunology · 2013Article
- Mouse models in male fertility research.Asian journal of andrology · 2011Review
- The effect on intracytoplasmic sperm injection outcome of genotype, male germ cell stage and freeze-thawing in mice.PloS one · 2010Article
Corrections and comments
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Authors and funding
13 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundIn laboratory mice and rats, congenic breeding is essential for analyzing the genes of interest on specific genetic backgrounds and for analyzing quantitative trait loci. However, in theory it takes about 3-4 years to achieve a strain carrying about 99% of the recipient genome at the tenth backcrossing (N10). Even with marker-assisted selection, the so-called 'speed congenic strategy', it takes more than a year at N4 or N5. METHODOLOGY/PRINCIPAL
findingsHere we describe a new high-speed congenic system using round spermatids retrieved from immature males (22-25 days of age). We applied the technique to three genetically modified strains of mice: transgenic (TG), knockin (KI) and N-ethyl-N-nitrosourea (ENU)-induced mutants. The donor mice had mixed genetic backgrounds of C57BL/6 (B6):DBA/2 or B6:129 strains. At each generation, males used for backcrossing were selected based on polymorphic marker analysis and their round spermatids were injected into B6 strain oocytes. Backcrossing was repeated until N4 or N5. For the TG and ENU-mutant strains, the N5 generation was achieved on days 188 and 190 and the proportion of B6-homozygous loci was 100% (74 markers) and 97.7% (172/176 markers), respectively. For the KI strain, N4 was achieved on day 151, all the 86 markers being B6-homozygous as early as on day 106 at N3. The carrier males at the final generation were all fertile and propagated the modified genes. Thus, three congenic strains were established through rapid generation turnover between 41 and 44 days. CONCLUSIONS/SIGNIFICANCE: This new high-speed breeding strategy enables us to produce congenic strains within about half a year. It should provide the fastest protocol for precise definition of the phenotypic effects of genes of interest on desired genetic backgrounds.
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