ArticleAntimicrobial agents and chemotherapy2009
Antibiotic resistance in Pseudomonas aeruginosa strains with increased mutation frequency due to inactivation of the DNA oxidative repair system.
Article in Antimicrobial agents and chemotherapy, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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38 citing papers in PubMed, 98 citations in OpenAlex.
- Antimicrobial activity of a short guanidine mimic immobilised on contact lenses.Journal of materials science. Materials in medicine · 2026Article
- Mapping the oxidative landscape in cystic fibrosis: methodological frontiers and application.Frontiers in pharmacology · 2025Review
- The impact of antioxidant-ciprofloxacin combinations on the evolution of antibiotic resistance in Pseudomonas aeruginosa biofilms.NPJ biofilms and microbiomes · 2024Article
- Survival Strategies ofAntibiotics (Basel, Switzerland) · 2024Article
- The activity of antimicrobial peptoids against multidrug-resistant ocular pathogens.Contact lens & anterior eye : the journal of the British Contact Lens Association · 2024Article
- Oxidative stress induced by Etoposide anti-cancer chemotherapy drives the emergence of tumor-associated bacteria resistance to fluoroquinolones.Journal of advanced research · 2024Article
- Drug resistance and physiological roles of RND multidrug efflux pumps inMicrobiology (Reading, England) · 2023Article
- Overcoming Antibiotic Resistance with Novel Paradigms of Antibiotic Selection.Microorganisms · 2022Review
- Hypermutator strains of Pseudomonas aeruginosa reveal novel pathways of resistance to combinations of cephalosporin antibiotics and beta-lactamase inhibitors.PLoS biology · 2022Article
- Antimicrobial Coating: Tracheal Tube Application.International journal of nanomedicine · 2022Review
- Antibiotic Resistance in Pseudomonas.Advances in experimental medicine and biology · 2022Article
- The methylation-independent mismatch repair machinery inMicrobiology (Reading, England) · 2021Review
- Antimicrobial Resistance and Inorganic Nanoparticles.International journal of molecular sciences · 2021Review
- Genome evolution drives transcriptomic and phenotypic adaptation inMicrobial genomics · 2021Article
- Oxidative Stress Response inPathogens (Basel, Switzerland) · 2021Review
- Inhibitors of DNA Glycosylases as Prospective Drugs.International journal of molecular sciences · 2020Review
- Characterization of Hypermutator Pseudomonas aeruginosa Isolates from Patients with Cystic Fibrosis in Australia.Antimicrobial agents and chemotherapy · 2019Article
- Production of highly stable spray dried phage formulations for treatment of Pseudomonas aeruginosa lung infection.European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V · 2017Article
- Surface charge modification decreases Pseudomonas aeruginosa adherence in vitro and bacterial persistence in an in vivo implant model.The Laryngoscope · 2017Article
- Evolutionary diversification of Pseudomonas aeruginosa in an artificial sputum model.BMC microbiology · 2017Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The chronic Pseudomonas aeruginosa infection of the lungs of cystic fibrosis (CF) patients is characterized by the biofilm mode of growth and chronic inflammation dominated by polymorphonuclear leukocytes (PMNs). A high percentage of P. aeruginosa strains show high frequencies of mutations (hypermutators [HP]). P. aeruginosa is exposed to oxygen radicals, both those generated by its own metabolism and especially those released by a large number of PMNs in response to the chronic CF lung infection. Our work therefore focused on the role of the DNA oxidative repair system in the development of HP and antibiotic resistance. We have constructed and characterized mutT, mutY, and mutM mutants in P. aeruginosa strain PAO1. The mutT and mutY mutants showed 28- and 7.5-fold increases in mutation frequencies, respectively, over that for PAO1. These mutators had more oxidative DNA damage (higher levels of 7,8-dihydro-8-oxodeoxyguanosine) than PAO1 after exposure to PMNs, and they developed resistance to antibiotics more frequently. The mechanisms of resistance were increased beta-lactamase production and overexpression of the MexCD-OprJ efflux-pump. Mutations in either the mutT or the mutY gene were found in resistant HP clinical isolates from patients with CF, and complementation with wild-type genes reverted the phenotype. In conclusion, oxidative stress might be involved in the development of resistance to antibiotics. We therefore suggest the possible use of antioxidants for CF patients to prevent the development of antibiotic resistance.
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