ArticleThe Journal of biological chemistry2009
c-Myc-induced aberrant DNA synthesis and activation of DNA damage response in p300 knockdown cells.
Article in The Journal of biological chemistry, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT03325517 (Effect of Adenovirus E1A Oncogene on DNA Replication Dynamics), which is not on this map. Cited by 22 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Effect of Adenovirus E1A Oncogene on DNA Replication Dynamics
Who cites it
22 citing papers in PubMed, 34 citations in OpenAlex.
- Stability of c-Myc Protein in Early S Phase Is Regulated by the Interaction with PCNA.International journal of molecular sciences · 2026Article
- The therapeutic potential of RNA m(6)A in lung cancer.Cell communication and signaling : CCS · 2024Review
- High abundance of CDC45 inhibits cell proliferation through elevation of HSPA6.Cell proliferation · 2022Article
- Review
- MYC: a multipurpose oncogene with prognostic and therapeutic implications in blood malignancies.Journal of hematology & oncology · 2021Review
- Emerging impact of the long noncoding RNA MIR22HG on proliferation and apoptosis in multiple human cancers.Journal of experimental & clinical cancer research : CR · 2020Review
- The Cdk2-c-Myc-miR-571 Axis Regulates DNA Replication and Genomic Stability by Targeting Geminin.Cancer research · 2019Article
- DNA replication stress: oncogenes in the spotlight.Genetics and molecular biology · 2019Article
- Network-based integration of multi-omics data for prioritizing cancer genes.Bioinformatics (Oxford, England) · 2018Article
- Family matters: How MYC family oncogenes impact small cell lung cancer.Cell cycle (Georgetown, Tex.) · 2017Review
- Proteasomal Inhibition by Ixazomib Induces CHK1 and MYC-Dependent Cell Death in T-cell and Hodgkin Lymphoma.Cancer research · 2016Article
- Sp1 and c-Myc modulate drug resistance of leukemia stem cells by regulating survivin expression through the ERK-MSK MAPK signaling pathway.Molecular cancer · 2015Article
- Identification and pharmacological inactivation of the MYCN gene network as a therapeutic strategy for neuroblastic tumor cells.The Journal of biological chemistry · 2015Article
- Cdc6 cooperates with c-Myc to promote genome instability and epithelial to mesenchymal transition EMT in zebrafish.Oncotarget · 2014Article
- CBP and p300 acetylate PCNA to link its degradation with nucleotide excision repair synthesis.Nucleic acids research · 2014Article
- MYC and the control of DNA replication.Cold Spring Harbor perspectives in medicine · 2014Review
- Article
- E1a promotes c-Myc-dependent replicative stress: implications in glioblastoma radiosensitization.Cell cycle (Georgetown, Tex.) · 2014Article
- Adenovirus E1A oncogene induces rereplication of cellular DNA and alters DNA replication dynamics.Journal of virology · 2013Article
- Cdc45 is a critical effector of myc-dependent DNA replication stress.Cell reports · 2013Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
We previously showed that in quiescent cells, p300/CBP (CREB-binding protein)family coactivators repress c-myc and prevent premature induction of DNA synthesis. p300/CBP-depleted cells exit G(1) early and continue to accumulate in S phase but do not progress into G(2)/M, and eventually they die of apoptosis. Here, we show that the S-phase arrest in these cells is because of an intra-S-phase block. The inappropriate DNA synthesis that occurs as a result of forced expression of c-myc leads to the activation of the DNA damage response as evidenced by the phosphorylation of several checkpoint related proteins and the formation of foci containing gamma-H2AX. The activation of checkpoint response is related to the induction of c-myc, as the phosphorylation of checkpoint proteins can be reversed when cells are treated with a c-Myc inhibitor or when Myc synthesis is blocked by short hairpin RNA. Using the DNA fiber assay, we show that in p300-depleted cells initiation of replication occurs from multiple replication origins. Chromatin loading of the Cdc45 protein also indicates increased origin activity in p300 knockdown cells. Immunofluorescence experiments indicate that c-Myc colocalizes with replication foci, consistent with the recently reported direct role of c-Myc in the initiation of DNA synthesis. Thus, the inappropriate S-phase entry of p300 down-regulated cells is likely to be because of c-Myc-induced deregulated replication origin activity, which results in replicative stress, activation of a DNA damage response, and S-phase arrest. Our results point to an important role for p300 in maintaining genomic integrity by negatively regulating c-myc.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.