ArticleThe journal of histochemistry and cytochemistry : official journal of the Histochemistry Society2009
Quantitative in situ detection of phosphoproteins in fixed tissues using quantum dot technology.
Article in The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed, 20 citations in OpenAlex.
- Advances in the application of nanotechnology in the diagnosis and treatment of gastrointestinal tumors.Molecular and clinical oncology · 2015Article
- Multiplexed ion beam imaging of human breast tumors.Nature medicine · 2014Article
- Proteomic analysis of the nuclear phosphorylated proteins in dairy cow mammary epithelial cells treated with estrogen.In vitro cellular & developmental biology. Animal · 2012Article
- Probing intracellular biomarkers and mediators of cell activation using nanosensors and bioorthogonal chemistry.ACS nano · 2011Article
- Protein kinase C-beta gene variants, pathway activation, and enzastaurin activity in lung cancer.Clinical lung cancer · 2010Article
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Authors and funding
3 authors at 1 institution in 1 country.
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Abstract
Detection and quantitation of phosphoproteins (PPs) in fixed tissues will become increasingly important as additional inhibitors of protein kinases enter clinical use and new disease entities are defined by molecular changes affecting PP levels. We characterize fixation conditions suitable for accurate PP quantitation that are achievable in a clinical laboratory and illustrate the utility of in situ quantitation of PPs by quantum dot (QD) nanocrystals in two models: (1) a therapeutic model demonstrating effects of a targeted therapeutic (quantitative reduction of phospho-GSK3beta) in xenografts treated with enzastaurin; and (2) a diagnostic model that identifies elevated levels of nuclear phospho-STAT5 in routine bone marrow biopsies from patients with acute myeloid leukemia based on the presence of the activating FLT3-ITD mutation. Finally, we document production of a well-characterized tissue microarray of widely available cell lines as a multilevel calibrator for validating numerous phosphoprotein assays. QD immunofluorescence is an ideal method for in situ quantitation of PPs in fixed samples, providing valuable cell type-specific and subcellular information about pathway activation in primary tissues.
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Registered trials
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