ArticleBMC genomics2009
Sequencing strategy for the whole mitochondrial genome resulting in high quality sequences.
Article in BMC genomics, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed.
- Changes inBiomedicines · 2024Article
- The effect of library preparation protocol on the efficiency of heteroplasmy detection in mitochondrial DNA using two massively parallel sequencing Illumina systems.Journal of applied genetics · 2024Article
- Phylogenetic insights into the genetic legacies of Hungarian-speaking communities in the Carpathian Basin.Scientific reports · 2024Article
- A PCR-independent approach for mtDNA enrichment and next-generation sequencing: comprehensive evaluation and clinical application.Journal of translational medicine · 2024Article
- POLG2-Linked Mitochondrial Disease: Functional Insights from New Mutation Carriers and Review of the Literature.Cerebellum (London, England) · 2024Review
- Assessment of Amikacin- and Capreomycin-Related Adverse Drug Reactions in Patients with Multidrug-Resistant Tuberculosis and Exploring the Role of Genetic Factors.Journal of personalized medicine · 2023Article
- Article
- Helena's Many Daughters: More Mitogenome Diversity behind the Most Common West Eurasian mtDNA Control Region Haplotype in an Extended Italian Population Sample.International journal of molecular sciences · 2022Article
- Use of Next-Generation Sequencing for Identifying Mitochondrial Disorders.Current issues in molecular biology · 2022Review
- Evidence for multi-copy Mega-NUMTs in the human genome.Nucleic acids research · 2021Article
- Analyzing Low-Level mtDNA Heteroplasmy-Pitfalls and Challenges from Bench to Benchmarking.International journal of molecular sciences · 2021Article
- Complete mitogenome data for the Serbian population: the contribution to high-quality forensic databases.International journal of legal medicine · 2020Article
- Genomic Landscape of the Mitochondrial Genome in the United Arab Emirates Native Population.Genes · 2020Article
- Profiling of Mitochondrial DNA Heteroplasmy in a Prospective Oral Squamous Cell Carcinoma Study.Cancers · 2020Article
- Detection of Innate and Artificial Mitochondrial DNA Heteroplasmy by Massively Parallel Sequencing: Considerations for Analysis.Journal of Korean medical science · 2018Article
- Mitochondrial genomes uncover the maternal history of the Pamir populations.European journal of human genetics : EJHG · 2018Article
- Entire Mitochondrial DNA Sequencing on Massively Parallel Sequencing for the Korean Population.Journal of Korean medical science · 2017Article
- Mapping human dispersals into the Horn of Africa from Arabian Ice Age refugia using mitogenomes.Scientific reports · 2016Article
- Oxidized mitochondrial nucleoids released by neutrophils drive type I interferon production in human lupus.The Journal of experimental medicine · 2016Article
- Simultaneous Whole Mitochondrial Genome Sequencing with Short Overlapping Amplicons Suitable for Degraded DNA Using the Ion Torrent Personal Genome Machine.Human mutation · 2015Article
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundIt has been demonstrated that a reliable and fail-safe sequencing strategy is mandatory for high-quality analysis of mitochondrial (mt) DNA, as the sequencing and base-calling process is prone to error. Here, we present a high quality, reliable and easy handling manual procedure for the sequencing of full mt genomes that is also appropriate for laboratories where fully automated processes are not available.
resultsWe amplified whole mitochondrial genomes as two overlapping PCR-fragments comprising each about 8500 bases in length. We developed a set of 96 primers that can be applied to a (manual) 96 well-based technology, which resulted in at least double strand sequence coverage of the entire coding region (codR).
conclusionThis elaborated sequencing strategy is straightforward and allows for an unambiguous sequence analysis and interpretation including sometimes challenging phenomena such as point and length heteroplasmy that are relevant for the investigation of forensic and clinical samples.
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