ArticleJournal of neurotrauma2009
Mechanically induced reactive gliosis causes ATP-mediated alterations in astrocyte stiffness.
Article in Journal of neurotrauma, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 56 citations in OpenAlex.
- Mechanobiology of the blood-brain barrier during development, disease and ageing.Nature communications · 2025Review
- IOP and glaucoma damage: The essential role of optic nerve head and retinal mechanosensors.Progress in retinal and eye research · 2024Review
- Inhibition of P2X4 and P2X7 receptors improves histological and behavioral outcomes after experimental traumatic brain injury in rats.Experimental and therapeutic medicine · 2023Article
- Commonly Overlooked Factors in Biocompatibility Studies of Neural Implants.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Review
- Hippocampus of the APPFrontiers in aging neuroscience · 2023Article
- P2Y1 Receptor as a Catalyst of Brain Neurodegeneration.NeuroSci · 2022Review
- A multilayer network model of neuron-astrocyte populations in vitro reveals mGluRNetwork neuroscience (Cambridge, Mass.) · 2022Article
- Mechanotransduction assays for neural regeneration strategies: A focus on glial cells.APL bioengineering · 2021Review
- Viscoelastic properties of white and gray matter-derived microglia differentiate upon treatment with lipopolysaccharide but not upon treatment with myelin.Journal of neuroinflammation · 2021Article
- Mechanosensation in traumatic brain injury.Neurobiology of disease · 2021Review
- Bioprinting: From Tissue and Organ Development toChemical reviews · 2020Review
- Nanomechanics and Histopathology as Diagnostic Tools to Characterize Freshly Removed Human Brain Tumors.International journal of nanomedicine · 2020Article
- Quantifying the Local Mechanical Properties of Cells in a Fibrous Three-Dimensional Microenvironment.Biophysical journal · 2019Article
- Astrocyte Mechano-Activation by High-Rate Overpressure Involves Alterations in Structural and Junctional Proteins.Frontiers in neurology · 2019Article
- Mechanical elongation of astrocyte processes to create living scaffolds for nervous system regeneration.Journal of tissue engineering and regenerative medicine · 2017Article
- The soft mechanical signature of glial scars in the central nervous system.Nature communications · 2017Article
- Callosal dysfunction explains injury sequelae in a computational network model of axonal injury.Journal of neurophysiology · 2016Article
- Spinal Astrocytic Thrombospondin-4 Induced by Excitatory Neuronal Signaling Mediates Pain After Facet Capsule Injury.Annals of biomedical engineering · 2016Article
- Astrogliosis in a dish: substrate stiffness induces astrogliosis in primary rat astrocytes.RSC advances · 2016Article
- Rheological properties of cells measured by optical tweezers.BMC biophysics · 2016Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
Abstract
Reactive gliosis is a process triggered in astrocytes after traumatic injury, yet the exact consequences of gliosis on cellular survival and neural regenerative processes in the injured brain remain only partly understood. One recently discovered feature influencing neuronal growth and differentiation is the physical stiffness of the environment surrounding pioneering neurites. In this study, the mechanical properties of cultured cortical astrocytes are measured following a mechanical stretch injury that induces reactive gliosis. In mechanically injured cultures, there was a significant increase in glial fibrillary acidic protein (GFAP) immunoreactivity 24 h following a rapid, transient 15% strain. In these same cultures, astrocytes in the surrounding region--the "mechanical penumbra"--also exhibited increased GFAP immunoreactivity compared to naive cultures. Correlated with these changes in GFAP was a general softening of the non-nuclear regions of the astrocytes, both in the injured and penumbra cells, as measured by atomic force microscopy (AFM). The elastic modulus in naive cultures was observed to be 57.7+/-5.8 kPa in non-nuclear regions of naive cultures, while 24 h after injury the modulus was observed to be 26.4+/-4.9 kPa in the same region of injured cells. In the penumbra of injured cultures, the modulus was 23.7+/-3.6 kPa. Alterations in astrocyte stiffness in the area of injury and mechanical penumbra were ameliorated by pretreating cultures with a nonselective P2 receptor antagonist (PPADS). Since neuronal cells generally prefer softer substrates for growth and neurite extension, these findings may indicate that the mechanical characteristics of reactive astrocytes are favorable for neuronal recovery after traumatic brain injury.
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Registered trials
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