ArticleFunctional & integrative genomics2009
Alternative splicing, activation of cryptic exons and amino acid substitutions in carotenoid biosynthetic genes are associated with lutein accumulation in wheat endosperm.
Article in Functional & integrative genomics, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
46 citing papers in PubMed, 122 citations in OpenAlex.
- Gene-Editing-Mediated Enhancement of Carotenoid Compound Accumulation in Common Wheat Grains.Foods (Basel, Switzerland) · 2026Article
- Article
- Genome-wide association study and linkage mapping reveal TaqW-6B associated with water-extractable arabinoxylan content in wheat grain.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2024Article
- Nudix hydrolase 23 post-translationally regulates carotenoid biosynthesis in plants.The Plant cell · 2024Article
- Marker-Trait Associations for Total Carotenoid Content and Individual Carotenoids in Durum Wheat Identified by Genome-Wide Association Analysis.Plants (Basel, Switzerland) · 2022Article
- Mutant combinations of lycopene ɛ-cyclase and β-carotene hydroxylase 2 homoeologs increased β-carotene accumulation in endosperm of tetraploid wheat (Triticum turgidum L.) grains.Plant biotechnology journal · 2022Article
- Phytoene Synthase: The Key Rate-Limiting Enzyme of Carotenoid Biosynthesis in Plants.Frontiers in plant science · 2022Review
- Overexpression ofFrontiers in plant science · 2022Article
- Alternative Splicing ofInternational journal of molecular sciences · 2021Article
- Genome wide association study of the whiteness and colour related traits of flour and dough sheets in common wheat.Scientific reports · 2021Article
- Durum Wheat (Foods (Basel, Switzerland) · 2021Article
- Transcription of lncRNARNA biology · 2020Article
- Multifamily QTL analysis and comprehensive design of genotypes for high-quality soft wheat.PloS one · 2020Article
- A GDSL Esterase/Lipase Catalyzes the Esterification of Lutein in Bread Wheat.The Plant cell · 2019Article
- Carotenoid Pigment Content in Durum Wheat (Frontiers in plant science · 2019Review
- Article
- A Genome-Wide Association Study Reveals a Rich Genetic Architecture of Flour Color-Related Traits in Bread Wheat.Frontiers in plant science · 2018Article
- Hybrid incompatibilities in interspecific crosses between tetraploid wheat and its wild diploid relative Aegilops umbellulata.Plant molecular biology · 2017Article
- The carotenoid biosynthetic and catabolic genes in wheat and their association with yellow pigments.BMC genomics · 2017Article
- Regulation of Banana Phytoene Synthase (MaPSY) Expression, Characterization and Their Modulation under Various Abiotic Stress Conditions.Frontiers in plant science · 2017Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Endosperm carotenoid content in wheat is a primary determinant of flour colour and this affects both the nutritional value of the grain and its utility for different applications. Utilising wheat rice synteny two genes, epsilon-cyclase (epsilon-LCY) and phytoene synthase (Psy-A1), were identified as candidate genes for two of the QTL affecting lutein content in wheat endosperm. Analysis of the sequence changes in epsilon-LCY and Psy-A1 revealed possible causal mechanisms for both QTL. A point mutation in epsilon-LCY results in the substitution of a conserved amino acid in the high lutein allele. This substitution has been observed in high lutein-accumulating species from the Gentiales order. In Psy-A1, a sequence duplication at the end of exon 2 creates a new splice site and causes alternative splicing of the transcript and activation of a cryptic exon, resulting in four different transcripts: a wild-type transcript, two transcripts with early terminations and a transcript that would produce an in-frame, albeit longer protein. Only the wild-type splice variant produced an enzymatically active protein and its mRNA abundance was reduced by titration with the other splice variants. This reduction in wild-type mRNA is argued to result in a reduction in PSY protein and thus carotenoid content in wheat.
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