ArticlePloS one2009
A raft-associated species of phosphatidylethanolamine interacts with cholesterol comparably to sphingomyelin. A Langmuir-Blodgett monolayer study.
Article in PloS one, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 44 citations in OpenAlex.
- Milk Fat Globule Membrane Is Associated with Lower Blood Lipid Levels in Adults: A Meta-Analysis of Randomized Controlled Trials.Foods (Basel, Switzerland) · 2024Article
- Varying the position of phospholipid acyl chain unsaturation modulates hopanoid and sterol ordering.Biophysical journal · 2024Article
- Is cholesterol both the lock and key to abnormal transmembrane signals in Autism Spectrum Disorder?Lipids in health and disease · 2024Article
- The Chemical Reactivity of Membrane Lipids.Chemical reviews · 2024Review
- Varying the position of phospholipid acyl chain unsaturation modulates hopanoid and sterol ordering.bioRxiv : the preprint server for biology · 2024Article
- Methodological Pitfalls of Investigating Lipid Rafts in the Brain: What Are We Still Missing?Biomolecules · 2024Review
- Statin Action Targets Lipid Rafts of Cell Membranes: GIXD/PM-IRRAS Investigation of Langmuir Monolayers.The journal of physical chemistry. B · 2023Article
- The Effect of Selected Flavonoids and Lipoic Acid on Natural and Model Cell Membranes: Langmuir and Microelectrophoretic Methods.Molecules (Basel, Switzerland) · 2023Article
- Simple Does Not Mean Trivial: Behavior of Phosphatidic Acid in Lipid Mono- and Bilayers.International journal of molecular sciences · 2021Article
- Designing a Useful Lipid Raft Model Membrane for Electrochemical and Surface Analytical Studies.Molecules (Basel, Switzerland) · 2021Article
- Building Blocks to Design Liposomal Delivery Systems.International journal of molecular sciences · 2020Review
- Review
- Model Plasma Membrane Exhibits a Microemulsion in Both Leaves Providing a Foundation for "Rafts".Biophysical journal · 2020Article
- The role of cholesterol and cholesterol-driven membrane raft domains in prostate cancer.Experimental biology and medicine (Maywood, N.J.) · 2019Review
- Caveolin-1 Endows Order in Cholesterol-Rich Detergent Resistant Membranes.Biomolecules · 2019Article
- Design of Therapeutic Self-Assembled Monolayers of Thiolated Abiraterone.Nanomaterials (Basel, Switzerland) · 2018Article
- Small-Molecule Modulation of Lipid-Dependent Cellular Processes against Cancer: Fats on the Gunpoint.BioMed research international · 2018Review
- Hopanoids as functional analogues of cholesterol in bacterial membranes.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Egg phospholipids and cardiovascular health.Nutrients · 2015Review
- Lipid rafts alter the stability and activity of the cholera toxin A1 subunit.The Journal of biological chemistry · 2012Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundSpecific interactions between sphingomyelin (SM) and cholesterol (Ch) are commonly believed to play a key role in the formation of rafts in the biological membranes. A weakness of this model is the implication that these microdomains are confined to the outer bilayer leaflet. The cytoplasmic leaflet, which contains the bulk of phosphatidylethanolamine (PE), phosphatidylserine (PS) and phosphatidylinositol (PI), is thought also to harbour half of the membrane cholesterol. Moreover, SLPE (1-stearoyl-2-linoleoyl-sn-glycero-3-phosphatidyl-ethanolamine) has recently been shown to be enriched in isolated detergent-resistant membranes (DRM), and this enrichment was independent of the method of isolation of DRM. METHODOLOGY/PRINCIPAL
findingsHere we present quantitative evidence coming from Langmuir-Blodgett monolayer experiments that SLPE forms complex with Ch similar to that between SM and Ch. The energies of these interactions as calculated form the monolayer studies are highly negative. FRAP analysis showed that NBD-Ch recovery was similar in liposomes composed of DOPC/Ch SM or SLPE but not DPPE, providing further evidence that SLPE may form an l(o) phase in the presence of high Ch concentration. Experiments on the solubility of DOPC liposomes containing DPPE/Ch (1ratio1), SM/Ch (1ratio1) or SLPE/Ch (1ratio1) showed the presence of Triton X-100 insoluble floating fraction (TIFF) in the case of SM/Ch or SLPE/Ch but not in DPPE/Ch containing liposomes. Quantitative determination of particular lipid species in the TIFF fraction confirms the conclusion that SLPE (or similar PE species) could be an important constituent of the inner leaflet raft.
conclusionSuch interactions suggest a possible existence of inner-leaflet nanoscale assemblies composed of cholesterol complexes with SLPE or similar unsaturated PE species.
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