ArticleApplied and environmental microbiology2009
Visual evidence of horizontal gene transfer between plants and bacteria in the phytosphere of transplastomic tobacco.
Article in Applied and environmental microbiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
24 citing papers in PubMed.
- Risk assessment of plant-to-bacterium transgene flow associated with novel small synthetic genome (minisynplastome) platforms for plastid genetic engineering.FEMS microbiology ecology · 2026Article
- Next-generation marker-free transplastomic plants: engineering the chloroplast genome without integration of marker genes in Solanum tuberosum (potato).Plant cell reports · 2024Article
- From grasslands to genes: exploring the major microbial drivers of antibiotic-resistance in microhabitats under persistent overgrazing.Microbiome · 2024Article
- Widespread horizontal gene transfer between plants and bacteria.ISME communications · 2024Article
- Plastid engineering using episomal DNA.Plant cell reports · 2023Review
- Article
- Horizontal gene transfer from genetically modified plants - Regulatory considerations.Frontiers in bioengineering and biotechnology · 2022Article
- Marker-Free Transplastomic Plants by Excision of Plastid Marker Genes Using Directly Repeated DNA Sequences.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Horizontal Gene Transfer and Endophytes: An Implication for the Acquisition of Novel Traits.Plants (Basel, Switzerland) · 2020Review
- Review
- Article
- Physicochemical Factors That Favor Conjugation of an Antibiotic Resistant Plasmid in Non-growing Bacterial Cultures in the Absence and Presence of Antibiotics.Frontiers in microbiology · 2018Article
- Discovery of Novel Plant Interaction Determinants from the Genomes of 163 Root Nodule Bacteria.Scientific reports · 2015Article
- Spatial scales of interactions among bacteria and between bacteria and the leaf surface.FEMS microbiology ecology · 2015Article
- Horizontal gene transfer in plants.Functional & integrative genomics · 2014Review
- Detecting rare gene transfer events in bacterial populations.Frontiers in microbiology · 2014Review
- Human Health Risk Assessment (HHRA) for environmental development and transfer of antibiotic resistance.Environmental health perspectives · 2013Review
- Assessing the probability of detection of horizontal gene transfer events in bacterial populations.Frontiers in microbiology · 2012Article
- Molecular-based environmental risk assessment of three varieties of genetically engineered cows.Transgenic research · 2011Article
- Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Plant surfaces, colonized by numerous and diverse bacterial species, are often considered hot spots for horizontal gene transfer (HGT) between plants and bacteria. Plant DNA released during the degradation of plant tissues can persist and remain biologically active for significant periods of time, suggesting that soil or plant-associated bacteria could be in direct contact with plant DNA. In addition, nutrients released during the decaying process may provide a copiotrophic environment conducive for opportunistic microbial growth. Using Acinetobacter baylyi strain BD413 and transplastomic tobacco plants harboring the aadA gene as models, the objective of this study was to determine whether specific niches could be shown to foster bacterial growth on intact or decaying plant tissues, to develop a competence state, and to possibly acquire exogenous plant DNA by natural transformation. Visualization of HGT in situ was performed using A. baylyi strain BD413(rbcL-DeltaPaadA::gfp) carrying a promoterless aadA::gfp fusion. Both antibiotic resistance and green fluorescence phenotypes were restored in recombinant bacterial cells after homologous recombination with transgenic plant DNA. Opportunistic growth occurred on decaying plant tissues, and a significant proportion of the bacteria developed a competence state. Quantification of transformants clearly supported the idea that the phytosphere constitutes a hot spot for HGT between plants and bacteria. The nondisruptive approach used to visualize transformants in situ provides new insights into environmental factors influencing HGT for plant tissues.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.