ArticleJournal of bacteriology2009
The Pseudomonas aeruginosa pyochelin-iron uptake pathway and its metal specificity.
Article in Journal of bacteriology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 82 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
82 citing papers in PubMed, 209 citations in OpenAlex.
- Article
- Bacterial Siderophore Production in Metal-Rich Environments: Underexplored Sources of Siderophores and Insights into Bioremediation.Journal of natural products · 2026Review
- Loss of the ferripyochelin receptor FptA drives reduced cefiderocol susceptibility and impairs fitness inAntimicrobial agents and chemotherapy · 2026Article
- A new family of TonB-dependent copper transporters linked to respiratory oxidase function.The Journal of biological chemistry · 2026Article
- Computational mass spectrometry and genome mining guided discovery of metallophores produced by Microbulbifer.ISME communications · 2026Article
- Review
- Forging biofilms: metal-induced microbial responses in biofilm formation.Journal of bacteriology · 2025Review
- Pseudomonas gelidaquae sp. nov., an Antarctic bacterium with a distinctive Type III Secretion System, isolated from Fildes Bay, King George Island.Antonie van Leeuwenhoek · 2025Article
- Loss of the Ferripyochelin Receptor FptA Drives Reduced Cefiderocol Susceptibility and Impairs Fitness inbioRxiv : the preprint server for biology · 2025Article
- Proteomic profiling of zinc homeostasis mechanisms in Pseudomonas aeruginosa through data-dependent and data-independent acquisition mass spectrometry.Metallomics : integrated biometal science · 2025Article
- Bacteria Under Metal Stress-Molecular Mechanisms of Metal Tolerance.International journal of molecular sciences · 2025Review
- The MDA-MB-231 Breast Cancer Cell Secretomes Modify Metabolomes ofInternational journal of molecular sciences · 2025Article
- Calprotectin elicits aberrant iron starvation responses inJournal of bacteriology · 2025Article
- Proteomic profiling of zinc homeostasis mechanisms inbioRxiv : the preprint server for biology · 2025Article
- Plant growth-promotion triggered by extracellular polymer is associated with facilitation of bacterial cross-feeding networks of the rhizosphere.The ISME journal · 2025Article
- Regulation of plant Ni uptake by soil-borne microorganisms occurs independently of their Ni-solubilizing capabilities.The ISME journal · 2025Article
- Article
- Bismuth-based drugs sensitize Pseudomonas aeruginosa to multiple antibiotics by disrupting iron homeostasis.Nature microbiology · 2024Article
- Relationship between Pyochelin andInternational journal of molecular sciences · 2024Review
- Plants, Microorganisms and Their Metabolites in Supporting Asbestos Detoxification-A Biological Perspective in Asbestos Treatment.Materials (Basel, Switzerland) · 2024Review
22 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Pyochelin (Pch) is one of the two major siderophores produced and secreted by Pseudomonas aeruginosa PAO1 to assimilate iron. It chelates iron in the extracellular medium and transports it into the cell via a specific outer membrane transporter, FptA. We used the fluorescent properties of Pch to show that this siderophore chelates, in addition to Fe(3+) albeit with substantially lower affinities, Ag(+), Al(3+), Cd(2+), Co(2+), Cr(2+), Cu(2+), Eu(3+), Ga(3+), Hg(2+), Mn(2+), Ni(2+), Pb(2+), Sn(2+), Tb(3+), Tl(+), and Zn(2+). Surprisingly, the Pch complexes with all these metals bound to FptA with affinities in the range of 10 nM to 4.8 microM (the affinity of Pch-Fe is 10 nM) and were able to inhibit, with various efficiencies, Pch-(55)Fe uptake in vivo. We used inductively coupled plasma atomic emission spectrometry to follow metal uptake by P. aeruginosa. Energy-dependent metal uptake, in the presence of Pch, was efficient only for Fe(3+). Co(2+), Ga(3+), and Ni(2+) were also transported, but the uptake rates were 23- to 35-fold lower than that for Fe(3+). No uptake was seen for all the other metals. Thus, cell surface FptA has broad metal specificity at the binding stage but is much more selective for the metal uptake process. This uptake pathway does not appear to efficiently assimilate any metal other than Fe(3+).
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.