ArticlePlant physiology2009
Identification and functional characterization of inhibitor-3, a regulatory subunit of protein phosphatase 1 in plants.
Article in Plant physiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 32 citations in OpenAlex.
- Novel Molecular Markers and Immune-Related Candidate Genes for Blackleg Resistance in Rapeseed: A Genome-Wide Analysis.International journal of molecular sciences · 2026Article
- PP1-associated responses linked to HMGR-related changes during postharvest squalene accumulation inFrontiers in plant science · 2026Article
- The role of forkhead-associated (FHA)-domain proteins in plant biology.Plant molecular biology · 2023Review
- Inhibitor-3 inhibits Protein Phosphatase 1 via a metal binding dynamic protein-protein interaction.Nature communications · 2023Article
- Rice protein phosphatase 1 regulatory subunits OsINH2 and OsINH3 participate actively in growth and adaptive responses under abscisic acid.Frontiers in plant science · 2022Article
- Role of Protein Phosphatase1 Regulatory Subunit3 in Mediating the Abscisic Acid Response.Plant physiology · 2020Article
- The Protein Phosphatases ATUNIS1 and ATUNIS2 Regulate Cell Wall Integrity in Tip-Growing Cells.The Plant cell · 2018Article
- EAR1 Negatively Regulates ABA Signaling by Enhancing 2C Protein Phosphatase Activity.The Plant cell · 2018Article
- Allyl-Isothiocyanate and Microcystin-LR Reveal the Protein Phosphatase Mediated Regulation of Metaphase-Anaphase Transition inFrontiers in plant science · 2018Article
- A Raf-like protein kinase BHP mediates blue light-dependent stomatal opening.Scientific reports · 2017Article
- Protein phosphatase 1 abrogates IRF7-mediated type I IFN response in antiviral immunity.European journal of immunology · 2016Article
- Type One Protein Phosphatase 1 and Its Regulatory Protein Inhibitor 2 Negatively Regulate ABA Signaling.PLoS genetics · 2016Article
- Arabidopsis DELLA protein degradation is controlled by a type-one protein phosphatase, TOPP4.PLoS genetics · 2014Article
- Revisiting the evolutionary history and roles of protein phosphatases with Kelch-like domains in plants.Plant physiology · 2014Article
- Article
- Plasmodium falciparum inhibitor-3 homolog increases protein phosphatase type 1 activity and is essential for parasitic survival.The Journal of biological chemistry · 2012Article
- Two ancient bacterial-like PPP family phosphatases from Arabidopsis are highly conserved plant proteins that possess unique properties.Plant physiology · 2011Article
- Indispensable Roles of Plastids in Arabidopsis thaliana Embryogenesis.Current genomics · 2010Article
- Phosphatidic acid inhibits blue light-induced stomatal opening via inhibition of protein phosphatase 1 [corrected].Plant physiology · 2010Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Protein phosphatase 1 (PP1) is a eukaryotic serine/threonine protein phosphatase, and mediates diverse cellular processes in animal systems via the association of a catalytic subunit (PP1c) with multiple regulatory subunits that determine the catalytic activity, the subcellular localization, and the substrate specificity. However, no regulatory subunit of PP1 has been identified in plants so far. In this study, we identified inhibitor-3 (Inh3) as a regulatory subunit of PP1 and characterized a functional role of Inh3 in Vicia faba and Arabidopsis (Arabidopsis thaliana). We found Inh3 as one of the proteins interacting with PP1c using a yeast two-hybrid system. Biochemical analyses demonstrated that Arabidopsis Inh3 (AtInh3) bound to PP1c via the RVxF motif of AtInh3, a consensus PP1c-binding sequence both in vitro and in vivo. AtInh3 inhibited the PP1c phosphatase activity in the nanomolar range in vitro. AtInh3 was localized in both the nucleus and cytoplasm, and it colocalized with Arabidopsis PP1c in these compartments. Disruption mutants of AtINH3 delayed the progression of early embryogenesis, arrested embryo development at the globular stage, and eventually caused embryo lethality. Furthermore, reduction of AtINH3 expression by RNA interference led to a decrease in fertility. Transformation of the lethal mutant of inh3 with wild-type AtINH3 restored the phenotype, whereas that with the AtINH3 gene having a mutation in the RVxF motif did not. These results define Inh3 as a regulatory subunit of PP1 in plants and suggest that Inh3 plays a crucial role in early embryogenesis in Arabidopsis.
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Registered trials
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