ArticlePlant physiology2009
In-depth proteome analysis of Arabidopsis leaf peroxisomes combined with in vivo subcellular targeting verification indicates novel metabolic and regulatory functions of peroxisomes.
Article in Plant physiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 109 papers.
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109 citing papers in PubMed, 257 citations in OpenAlex.
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- A non-canonical fungal peroxisome PTS-1 signal, SYM, and its evolutionary aspects.Scientific reports · 2025Article
- Global impacts of peroxisome and pexophagy dysfunction revealed through multi-omics analyses of lon2 and atg2 mutants.The Plant journal : for cell and molecular biology · 2024Article
- N-carboxyacyl and N-α-aminoacyl derivatives of aminoaldehydes as shared substrates of plant aldehyde dehydrogenases 10 and 7.Amino acids · 2024Article
- Evidence for dual targeting control of Arabidopsis 6-phosphogluconate dehydrogenase isoforms by N-terminal phosphorylation.Journal of experimental botany · 2024Article
- Visualizing the dynamics of plant energy organelles.Biochemical Society transactions · 2023Article
- ARP2/3 complex associates with peroxisomes to participate in pexophagy in plants.Nature plants · 2023Article
- Bioinformatic analysis of short-chain dehydrogenase/reductase proteins in plant peroxisomes.Frontiers in plant science · 2023Article
- Plant peroxisome proteostasis-establishing, renovating, and dismantling the peroxisomal proteome.Essays in biochemistry · 2022Review
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- Inferred Subcellular Localization of Peroxisomal Matrix Proteins ofFrontiers in plant science · 2022Article
- Peroxisomal Proteome Mining of Sweet Pepper (Frontiers in plant science · 2022Article
- In Silico Identification and Characterization of B12D Family Proteins in Viridiplantae.Evolutionary bioinformatics online · 2022Article
- Distribution and Functions of Monodehydroascorbate Reductases in Plants: Comprehensive Reverse Genetic Analysis ofAntioxidants (Basel, Switzerland) · 2021Article
- Peroxisomal PEX7 Receptor Affects Cadmium-Induced ROS and Auxin Homeostasis in Arabidopsis Root System.Antioxidants (Basel, Switzerland) · 2021Article
- Glutathione Metabolism in Plants under Stress: Beyond Reactive Oxygen Species Detoxification.Metabolites · 2021Review
- Nitric Oxide (NO) Scaffolds the Peroxisomal Protein-Protein Interaction Network in Higher Plants.International journal of molecular sciences · 2021Review
- Isolation and comparative proteomic analysis of mitochondria from the pulp of ripening citrus fruit.Horticulture research · 2021Article
- Citramalate synthase yields a biosynthetic pathway for isoleucine and straight- and branched-chain ester formation in ripening apple fruit.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
49 more citing papers are in PubMed but not listed here.
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12 authors at 2 institutions in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Peroxisomes are metabolically diverse organelles with essential roles in plant development. The major protein constituents of plant peroxisomes are well characterized, whereas only a few low-abundance and regulatory proteins have been reported to date. We performed an in-depth proteome analysis of Arabidopsis (Arabidopsis thaliana) leaf peroxisomes using one-dimensional gel electrophoresis followed by liquid chromatography and tandem mass spectrometry. We detected 65 established plant peroxisomal proteins, 30 proteins whose association with Arabidopsis peroxisomes had been previously demonstrated only by proteomic data, and 55 putative novel proteins of peroxisomes. We subsequently tested the subcellular targeting of yellow fluorescent protein fusions for selected proteins and confirmed the peroxisomal localization for 12 proteins containing predicted peroxisome targeting signals type 1 or 2 (PTS1/2), three proteins carrying PTS-related peptides, and four proteins that lack conventional targeting signals. We thereby established the tripeptides SLM> and SKV> (where > indicates the stop codon) as new PTS1s and the nonapeptide RVx(5)HF as a putative new PTS2. The 19 peroxisomal proteins conclusively identified from this study potentially carry out novel metabolic and regulatory functions of peroxisomes. Thus, this study represents an important step toward defining the complete plant peroxisomal proteome.
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