ArticleAmerican journal of physiology. Lung cellular and molecular physiology2009
Characterization of mouse alveolar epithelial cell monolayers.
Article in American journal of physiology. Lung cellular and molecular physiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 51 papers.
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Who cites it
51 citing papers in PubMed, 76 citations in OpenAlex.
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- Defining signals that promote human alveolar type I differentiation.American journal of physiology. Lung cellular and molecular physiology · 2024Article
- Human Pluripotent Stem Cell-Derived Alveolar Organoids: Cellular Heterogeneity and Maturity.Tuberculosis and respiratory diseases · 2024Article
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- Basal stem cell progeny establish their apical surface in a junctional niche during turnover of an adult barrier epithelium.Nature cell biology · 2023Article
- Activation of TREK-1 (American journal of physiology. Lung cellular and molecular physiology · 2023Article
- MICU1-dependent mitochondrial calcium uptake regulates lung alveolar type 2 cell plasticity and lung regeneration.JCI insight · 2022Article
- Regenerative medicine technologies applied to transplant medicine. An update.Frontiers in bioengineering and biotechnology · 2022Review
- Hedgehog interacting protein-expressing lung fibroblasts suppress lymphocytic inflammation in mice.JCI insight · 2021Article
- Type II alveolar epithelial cell-specific loss of RhoA exacerbates allergic airway inflammation through SLC26A4.JCI insight · 2021Article
- Conditioned Medium of Bone Marrow Mesenchymal Stem Cells Involved in Acute Lung Injury by Regulating Epithelial Sodium ChannelsFrontiers in bioengineering and biotechnology · 2021Article
- Genome-wide integration of microRNA and transcriptomic profiles of differentiating human alveolar epithelial cells.American journal of physiology. Lung cellular and molecular physiology · 2020Article
- Plasmin improves blood-gas barrier function in oedematous lungs by cleaving epithelial sodium channels.British journal of pharmacology · 2020Article
- Exophilin-5 regulates allergic airway inflammation by controlling IL-33-mediated Th2 responses.The Journal of clinical investigation · 2020Article
- Epithelial membrane protein 2 governs transepithelial migration of neutrophils into the airspace.The Journal of clinical investigation · 2020Article
- Lipopolysaccharide Inhibits Alpha Epithelial Sodium Channel Expression via MiR-124-5p in Alveolar Type 2 Epithelial Cells.BioMed research international · 2020Article
- Optimizing theJournal of stem cells & regenerative medicine · 2020Article
- Article
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
Abstract
We investigated the influence of extracellular matrix on transport properties of mouse alveolar epithelial cell (AEC) monolayers (MAECM) and transdifferentiation of isolated mouse alveolar epithelial type II (AT2) cells into an alveolar epithelial type I (AT1) cell-like phenotype. Primary mouse AT2 cells plated on laminin 5-coated polycarbonate filters formed monolayers with transepithelial resistance (R(T)) and equivalent short-circuit current (I(EQ)) of 1.8 kOmega.cm(2) and 5.3 microA/cm(2), respectively, after 8 days in culture. Amiloride (10 microM), ouabain (0.1 mM), and pimozide (10 microM) decreased MAECM I(EQ) to 40%, 10%, and 65% of its initial value, respectively. Sequential addition of pimozide and amiloride, in either order, revealed that their inhibitory effects are additive, suggesting that cyclic nucleotide-gated channels contribute to amiloride-insensitive active ion transport across MAECM. Ussing chamber measurements of unidirectional ion fluxes across MAECM under short-circuit conditions indicated that net absorption of Na(+) in the apical-to-basolateral direction is comparable to net ion flux calculated from the observed short-circuit current: 0.38 and 0.33 microeq.cm(-2).h(-1), respectively. Between days 1 and 9 in culture, AEC demonstrated increased expression of aquaporin-5 protein, an AT1 cell marker, and decreased expression of pro-surfactant protein-C protein, an AT2 cell marker, consistent with transition to an AT1 cell-like phenotype. These results demonstrate that AT1 cell-like MAECM grown on laminin 5-coated polycarbonate filters exhibit active and passive transport properties that likely reflect the properties of intact mouse alveolar epithelium. This mouse in vitro model will enhance the study of AEC derived from mutant strains of mice and help define important structure-function correlations.
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