ArticleFree radical biology & medicine2009
Inhibition of nitric oxide synthase by cobalamins and cobinamides.
Article in Free radical biology & medicine, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. It is linked to trial NCT03783091 (A Phase II Double Blind Randomized Controlled Trial of High Dose Vitamin B12 in Septic Shock), which is not on this map. Cited by 31 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
A Phase II Double Blind Randomized Controlled Trial of High Dose Vitamin B12 in Septic Shock
Who cites it
31 citing papers in PubMed, 1 synthesis or guideline pooled it, 76 citations in OpenAlex.
- Effects of different vitamins on individuals with septic shock: a Bayesian NMA of RCTs.Frontiers in nutrition · 2025Pooled it
- Efficacy of Treating Nifedipine-Induced Shock with Hydroxocobalamin in a Swine Model.Journal of medical toxicology : official journal of the American College of Medical Toxicology · 2025Article
- Association between low vitamin B-12 status and latent tuberculosis infection among adults.Asia Pacific journal of clinical nutrition · 2025Article
- Complete Immunophenotypic Reversal of Chronic Lymphocytic Leukaemia With High Dose Parenteral Methylcobalamin: A Case Report and Brief Review of Cobalamin in Cancer.Cancer reports (Hoboken, N.J.) · 2025Review
- Hydroxocobalamin for the treatment of vasoplegia after lung transplantation: A case series.JHLT open · 2025Article
- Methylcobalamin as a candidate for chronic peripheral neuropathic pain therapy: review of molecular pharmacology actiona.The Korean journal of pain · 2024Review
- Vitamin B12 status and the risk of developing sepsis in patients with bacterial infection: a prospective observational cohort study.BMC medicine · 2024Observational
- Comment on: "Methylene Blue-Induced Serotonin Toxicity: Case Files of the Medical Toxicology Fellowship at the New York City Poison Control Center".Journal of medical toxicology : official journal of the American College of Medical Toxicology · 2024Article
- Inverted U-shaped relationship between serum vitamin B12 and α-Klotho levels in US adults: a cross-sectional study.Frontiers in nutrition · 2024Article
- Antinociceptive effects of vitamin B-complex: A behavioral and histochemical study in rats.IBRO neuroscience reports · 2023Article
- Anti-Inflammatory and Vasorelaxant Effects Induced by an Aqueous Aged Black Garlic Extract Supplemented with Vitamins D, C, and B12 on Cardiovascular System.Foods (Basel, Switzerland) · 2023Article
- Vasoplegic syndrome in patients undergoing heart transplantation.Frontiers in surgery · 2023Article
- Vasoplegic Syndrome after Cardiopulmonary Bypass in Cardiovascular Surgery: Pathophysiology and Management in Critical Care.Journal of clinical medicine · 2022Review
- Hydroxocobalamin: An Effective Treatment for Flushing and Persistent Erythema in Rosacea.The Journal of clinical and aesthetic dermatology · 2022Article
- Early Use of Methylene Blue in Vasoplegic Syndrome: A 10-Year Propensity Score-Matched Cohort Study.Journal of clinical medicine · 2022Article
- Why intermolecular nitric oxide (NO) transfer? Exploring the factors and mechanistic aspects of NO transfer reaction.Chemical science · 2022Article
- Vitamin therapy in sepsis.Pediatric research · 2022Review
- Article
- Vasoplegic syndrome following cardiothoracic surgery-review of pathophysiology and update of treatment options.Critical care (London, England) · 2020Review
- Vasoplegia after cardiopulmonary bypass: A narrative review of pathophysiology and emerging targeted therapies.SAGE open medicine · 2020Review
Corrections and comments
- Erratum issued
Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
Cobalamins are important cofactors for methionine synthase and methylmalonyl-CoA mutase. Certain corrins also bind nitric oxide (NO), quenching its bioactivity. To determine if corrins would inhibit NO synthase (NOS), we measured their effects on -L-[(14)C]arginine-to-L-[(14)C]citrulline conversion by NOS1, NOS2, and NOS3. Hydroxocobalamin (OH-Cbl), cobinamide, and dicyanocobinamide (CN(2)-Cbi) potently inhibited all isoforms, whereas cyanocobalamin, methylcobalamin, and adenosylcobalamin had much less effect. OH-Cbl and CN(2)-Cbi prevented binding of the oxygen analog carbon monoxide (CO) to the reduced NOS1 and NOS2 heme active site. CN(2)-Cbi did not react directly with NO or CO. Spectral perturbation analysis showed that CN(2)-Cbi interacted directly with the purified NOS1 oxygenase domain. NOS inhibition by corrins was rapid and not reversed by dialysis with L-arginine or tetrahydrobiopterin. Molecular modeling indicated that corrins could access the unusually large heme- and substrate-binding pocket of NOS. Best fits were obtained in the "base-off" conformation of the lower axial dimethylbenzimidazole ligand. CN(2)-Cbi inhibited interferon-gamma-activated Raw264.7 mouse macrophage NO production. We show for the first time that certain corrins directly inhibit NOS, suggesting that these agents (or their derivatives) may have pharmacological utility. Endogenous cobalamins and cobinamides might play important roles in regulating NOS activity under normal and pathological conditions.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.