ArticleJournal of immunological methods2009
Subcellular fractionation of human eosinophils: isolation of functional specific granules on isoosmotic density gradients.
Article in Journal of immunological methods, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- Biological effects of leukotriene E4 on eosinophils.Prostaglandins, leukotrienes, and essential fatty acids · 2014Trial
- Neutral Buoyancy as a Simple Approach to Simulated Microgravity.Tissue engineering and regenerative medicine · 2026Article
- Emerging technologies towards extracellular vesicles large-scale production.Bioactive materials · 2025Review
- Extracellular sombrero vesicles are hallmarks of eosinophilic cytolytic degranulation in tissue sites of human diseases.Journal of leukocyte biology · 2024Article
- Biogenesis, Isolation, and Detection of Exosomes and Their Potential in Therapeutics and Diagnostics.Biosensors · 2023Review
- Eosinophilia in cancer and its regulation by sex hormones.Trends in endocrinology and metabolism: TEM · 2023Review
- Support of bone mineral deposition by regulation of pH.American journal of physiology. Cell physiology · 2018Article
- Functions of tissue-resident eosinophils.Nature reviews. Immunology · 2017Review
- Cysteinyl Leukotrienes in Eosinophil Biology: Functional Roles and Therapeutic Perspectives in Eosinophilic Disorders.Frontiers in medicine · 2017Review
- Isolation of human salivary extracellular vesicles by iodixanol density gradient ultracentrifugation and their characterizations.Journal of extracellular vesicles · 2016Article
- Purinergic P2Y12 Receptor Activation in Eosinophils and the Schistosomal Host Response.PloS one · 2015Article
- The new deal: a potential role for secreted vesicles in innate immunity and tumor progression.Frontiers in immunology · 2015Review
- Multifaceted roles of cysteinyl leukotrienes in eliciting eosinophil granule protein secretion.BioMed research international · 2015Review
- Article
- Eosinophil crystalloid granules: structure, function, and beyond.Journal of leukocyte biology · 2012Review
- CCL11 elicits secretion of RNases from mouse eosinophils and their cell-free granules.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2012Article
- MHC Class II and CD9 in human eosinophils localize to detergent-resistant membrane microdomains.American journal of respiratory cell and molecular biology · 2012Article
- Quantitative analysis of cell surface membrane proteins using membrane-impermeable chemical probe coupled with 18O labeling.Journal of proteome research · 2010Article
- Cysteinyl leukotrienes acting via granule membrane-expressed receptors elicit secretion from within cell-free human eosinophil granules.The Journal of allergy and clinical immunology · 2010Article
- Functional extracellular eosinophil granules: novel implications in eosinophil immunobiology.Current opinion in immunology · 2009Review
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
Subcellular fractionation has been an important tool in investigating human eosinophil structure and function, including localizing of cytokine/chemokines within granules, investigating granule protein translocation and intracellular transport during eosinophil secretion, and studying secretory mechanisms of granules. The resolution of organelles obtained by subcellular fractionation was improved considerably after the introduction of nonionic iodinated density-gradient metrizamide and Nycodenz media that, unlike sucrose, exhibit relatively low tonicity throughout the gradient. However, the structure and membrane preservation of isolated organelles were still compromised due to the lack of gradient isoosmolarity. This paper describes a detailed protocol of subcellular fractionation of nitrogen cavitated eosinophils on an isoosmotic iodinated density gradient (iodixanol - OptiPrep) and the isolation of well preserved and functional membrane-bound specific granules.
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Registered trials
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