ArticleJournal of immunological methods2009
A streamlined method for rapid and sensitive chromatin immunoprecipitation.
Article in Journal of immunological methods, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
10 citing papers in PubMed.
- Transcriptional Regulation of the Human IL5RA Gene through Alternative Promoter Usage during Eosinophil Development.International journal of molecular sciences · 2021Article
- A comparative study of ChIP-seq sequencing library preparation methods.BMC genomics · 2016Article
- Efficient chromatin immunoprecipitation using limiting amounts of biomass.Journal of visualized experiments : JoVE · 2013Article
- ERK2-mediated phosphorylation of transcriptional coactivator binding protein PIMT/NCoA6IP at Ser298 augments hepatic gluconeogenesis.PloS one · 2013Article
- Limitations and possibilities of low cell number ChIP-seq.BMC genomics · 2012Article
- DNA double-strand breaks relieve USF-mediated repression of Dβ2 germline transcription in developing thymocytes.Journal of immunology (Baltimore, Md. : 1950) · 2012Article
- Dissecting epigenetic silencing complexity in the mouse lung cancer suppressor gene Cadm1.PloS one · 2012Article
- Fur negatively regulates hns and is required for the expression of HilA and virulence in Salmonella enterica serovar Typhimurium.Journal of bacteriology · 2011Article
- Tetra-O-methyl nordihydroguaiaretic acid (Terameprocol) inhibits the NF-κB-dependent transcription of TNF-α and MCP-1/CCL2 genes by preventing RelA from binding its cognate sites on DNA.Journal of inflammation (London, England) · 2010Article
- Promoter activity 5' of Dbeta2 is coordinated by E47, Runx1, and GATA-3.Molecular immunology · 2009Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
We report a streamlined procedure to efficiently carry samples from chromatin to qPCR-compatible DNA in as little as 4 h. We use this streamlined ChIP to quantify histone H3 modifications at active (cad) and repressed (T early alpha) promoters in a Rag1-deficient pro-T cell line after 1-2 h IP. We further show that the protocol readily quantified histone modifications in chromatin from 10(4) Rag-deficient DN thymocytes. Taken together, these data outline a simple, cost-effective procedure for efficient ChIP analysis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.