ArticleBiosensors & bioelectronics2009
Single chain fragment variable recombinant antibody functionalized gold nanoparticles for a highly sensitive colorimetric immunoassay.
Article in Biosensors & bioelectronics, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed.
- Single Radial Immunodiffusion (SRID) Assay for Quantitative Determination of Recombinant SOD Protein in a Brucellosis Vaccine Candidate: Method Development and Validation.Methods and protocols · 2026Article
- Rapid and Convenient Single-Chain Variable Fragment-Employed Electrochemical C-Reactive Protein Detection System.International journal of molecular sciences · 2024Article
- Dopamine DBiomolecules · 2022Article
- A Novel Full-length IgG Recombinant Antibody Highly Specific to Clothianidin and Its Application in Immunochromatographic Assay.Biosensors · 2022Article
- A colorimetric sandwich-type bioassay for SARS-CoV-2 using a hACE2-based affinity peptide pair.Journal of hazardous materials · 2022Article
- Nanobiosensor Based on Sugar Code-AuNPs Aggregation: A Key to Opening New Gates in Rapid Diagnosis of Streptococcal Pharyngitis.Frontiers in bioengineering and biotechnology · 2022Article
- Targeted Codelivery of Prodigiosin and Simvastatin Using Smart BioMOF: Functionalization by Recombinant Anti-VEGFR1 scFv.Frontiers in bioengineering and biotechnology · 2022Article
- Monolayer Sensitivity Enables a 2D IR Spectroscopic Immuno-biosensor for Studying Protein Structures: Application to Amyloid Polymorphs.The journal of physical chemistry letters · 2019Article
- Modular Protein Engineering Approach to the Functionalization of Gold Nanoparticles for Use in Clinical Diagnostics.ACS applied nano materials · 2018Article
- Development and Comparison of Two Immuno-disaggregation Based Bioassays for Cell Secretome Analysis.Theranostics · 2018Article
- Article
- Gold nanoparticle contrast agents in advanced X-ray imaging technologies.Molecules (Basel, Switzerland) · 2013Review
- Targeted multiplex imaging mass spectrometry with single chain fragment variable (scfv) recombinant antibodies.Journal of the American Society for Mass Spectrometry · 2012Article
- Recombinant antibodies and their use in biosensors.Analytical and bioanalytical chemistry · 2012Review
- Enhancing nanoparticle-based visible detection by controlling the extent of aggregation.Scientific reports · 2012Article
- Functionalized Gold Nanoparticles and Their Biomedical Applications.Nanomaterials (Basel, Switzerland) · 2011Review
- Superparamagnetic iron oxide nanotheranostics for targeted cancer cell imaging and pH-dependent intracellular drug release.Molecular pharmaceutics · 2010Article
- Multivalent interaction-based carbohydrate biosensors for signal amplification.Biosensors & bioelectronics · 2010Article
- Colorimetric immunoassay for detection of tumor markers.International journal of molecular sciences · 2010Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
In this report, the peptide linker connecting scFv V(H) and V(L) domains were genetically modified to contain different amino acids (i.e. cysteine (scFv-cys) or histidines (scFv-his)) to enable the scFv to adsorb or self-assemble onto the gold nanoparticles (NPs). The scFv-cys stabilized gold NPs were used to develop a highly sensitive colorimetric immunosensor. The scFv-cys stabilized gold NPs were characterized by UV-vis spectra, transmission electron microscope (TEM) and FTIR. After adding the antigen rabbit IgG, the solution of scFv-cys stabilized gold NPs shows obvious visible color change from deep red to light purple due to the aggregation of the gold nanoparticles. Based on the colorimetric aggregation of scFv-cys stabilized gold NPs, the immunosensor exhibits high sensitivity with a detection limit of 1.7 nM and good specificity. The good properties of the colorimetric aggregation immunosensor can be attributed to the small size of scFv and the covalent link between the scFv and gold NPs that improve the better orientation and enhance the probe density. With the advantages of speed, simplicity and specificity, the colorimetric immunoassay based on the functionalized scFv stabilized gold NPs represents a promising approach for protein analysis and clinical diagnostics.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.