ArticleInternational journal for parasitology2009
Identification of an Ascaris G protein-coupled acetylcholine receptor with atypical muscarinic pharmacology.
Article in International journal for parasitology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 31 citations in OpenAlex.
- Two types of axonal muscarinic acetylcholine receptors mediate formation of saliva cocktail in the tick Ixodes ricinus.Nature communications · 2026Article
- Functional characterization of three G protein-coupled acetylcholine receptors in parasitic nematode Trichinella spiralis.International journal for parasitology. Drugs and drug resistance · 2023Article
- Pharmacological Profiling of a Brugia malayi Muscarinic Acetylcholine Receptor as a Putative Antiparasitic Target.Antimicrobial agents and chemotherapy · 2023Article
- Cholinergic receptors on intestine cells of Ascaris suum and activation of nAChRs by levamisole.International journal for parasitology. Drugs and drug resistance · 2020Article
- Prospects and challenges of CRISPR/Cas genome editing for the study and control of neglected vector-borne nematode diseases.The FEBS journal · 2016Review
- A constitutively active G protein-coupled acetylcholine receptor regulates motility of larval Schistosoma mansoni.Molecular and biochemical parasitology · 2015Article
- Functional expression and characterization of the C. elegans G-protein-coupled FLP-2 Receptor (T19F4.1) in mammalian cells and yeast.International journal for parasitology. Drugs and drug resistance · 2013Article
- Evidence for a bacterial lipopolysaccharide-recognizing G-protein-coupled receptor in the bacterial engulfment by Entamoeba histolytica.Eukaryotic cell · 2013Article
- Involvement of a putative intercellular signal-recognizing G protein-coupled receptor in the engulfment of Salmonella by the protozoan Tetrahymena.Open veterinary journal · 2013Article
- Parasite neuropeptide biology: Seeding rational drug target selection?International journal for parasitology. Drugs and drug resistance · 2012Review
- Biogenic amines and the control of neuromuscular signaling in schistosomes.Invertebrate neuroscience : IN · 2012Review
- A novel G protein-coupled receptor of Schistosoma mansoni (SmGPR-3) is activated by dopamine and is widely expressed in the nervous system.PLoS neglected tropical diseases · 2012Article
- Ion channels and receptor as targets for the control of parasitic nematodes.International journal for parasitology. Drugs and drug resistance · 2011Review
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
Abstract
Acetylcholine (ACh) is a neurotransmitter/neuromodulator in the nematode nervous system and induces its effects through interaction with both ligand-gated ion channels (LGICs) and G protein-coupled receptors (GPCRs). The structure, pharmacology and physiological importance of LGICs have been appreciably elucidated in model nematodes, including parasitic species where they are targets for anthelmintic drugs. Significantly less, however, is understood about nematode ACh GPCRs, termed GARs (G protein-linked ACh receptors). What is known comes from the free-living Caenorhabditis elegans as no GARs have been characterized from parasitic species. Here we clone a putative GAR from the pig gastrointestinal nematode Ascaris suum with high structural homology to the C. elegans receptor GAR-1. Our GPCR, dubbed AsGAR-1, is alternatively spliced and expressed in the head and tail of adult worms but not in dorsal or ventral body wall muscle, or the ovijector. ACh activated AsGAR-1 in a concentration-dependent manner but the receptor was not activated by other small neurotransmitters. The classical muscarinic agonists carbachol, arecoline, oxotremorine M and bethanechol were also AsGAR-1 agonists but pilocarpine was ineffective. AsGAR-1 activation by ACh was partially antagonized by the muscarinic blocker atropine but pirenzepine and scopolamine were largely ineffective. Certain biogenic amine GPCR antagonists were also found to block AsGAR-1. Our conclusion is that Ascaris possesses G protein-coupled ACh receptors that are homologous in structure to those present in C. elegans, and that although they have some sequence homology to vertebrate muscarinic receptors, their pharmacology is atypically muscarinic.
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Registered trials
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