ArticleBMC biotechnology2009
AC133+ progenitor cells as gene delivery vehicle and cellular probe in subcutaneous tumor models: a preliminary study.
Article in BMC biotechnology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
18 citing papers in PubMed, 32 citations in OpenAlex.
- Tracking of Labelled Stem Cells Using Molecular MR Imaging in a Mouse Burn ModelAdvances in molecular imaging · 2021Article
- Radionuclide-fluorescence Reporter Gene Imaging to Track Tumor Progression in Rodent Tumor Models.Journal of visualized experiments : JoVE · 2018Article
- Imaging techniques: new avenues in cancer gene and cell therapy.Cancer gene therapy · 2017Review
- A whole-body dual-modality radionuclide optical strategy for preclinical imaging of metastasis and heterogeneous treatment response in different microenvironments.Journal of nuclear medicine : official publication, Society of Nuclear Medicine · 2014Article
- Monitoring adenoviral based gene delivery in rat glioma by molecular imaging.World journal of clinical oncology · 2013Article
- Non-invasive molecular imaging for preclinical cancer therapeutic development.British journal of pharmacology · 2013Review
- Tracking of In-111-labeled human umbilical tissue-derived cells (hUTC) in a rat model of cerebral ischemia using SPECT imaging.BMC medical imaging · 2012Article
- Cord blood endothelial progenitor cells as therapeutic and imaging probes.Imaging in medicine · 2012Article
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- Image-guided, tumor stroma-targeted 131I therapy of hepatocellular cancer after systemic mesenchymal stem cell-mediated NIS gene delivery.Molecular therapy : the journal of the American Society of Gene Therapy · 2011Article
- Mesenchymal Stem Cell-mediated delivery of the sodium iodide symporter supports radionuclide imaging and treatment of breast cancer.Stem cells (Dayton, Ohio) · 2011Article
- Concise review: Mesenchymal stem cell tumor-homing: detection methods in disease model systems.Stem cells (Dayton, Ohio) · 2011Review
- Lentiviral Based Gene Transduction and Promoter Studies in Human Hematopoietic Stem Cells (hHSCs).Journal of stem cells & regenerative medicine · 2011Article
- The role and therapeutic potential of endothelial progenitor cells in tumor neovascularization.TheScientificWorldJournal · 2010Review
- Differentiation of glioma and radiation injury in rats using in vitro produce magnetically labeled cytotoxic T-cells and MRI.PloS one · 2010Article
- Non-invasive cell tracking in cancer and cancer therapy.Current topics in medicinal chemistry · 2010Review
- Application of Umbilical Cord Blood Derived Stem Cells in Diseases of the Nervous System.Journal of stem cell research & therapyArticle
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
Abstract
backgroundDespite enormous progress in gene therapy for breast cancer, an optimal systemic vehicle for delivering gene products to the target tissue is still lacking. The purpose of this study was to determine whether AC133+ progenitor cells (APC) can be used as both gene delivery vehicles and cellular probes for magnetic resonance imaging (MRI). In this study, we used superparamagentic iron oxide (SPIO)-labeled APCs to carry the human sodium iodide symporter (hNIS) gene to the sites of implanted breast cancer in mouse model. In vivo real time tracking of these cells was performed by MRI and expression of hNIS was determined by Tc-99m pertechnetate (Tc-99m) scan.
resultsThree million human breast cancer (MDA-MB-231) cells were subcutaneously implanted in the right flank of nude mice. APCs, isolated from fresh human cord blood, were genetically transformed to carry the hNIS gene using adenoviral vectors and magnetically labeled with ferumoxides-protamine sulfate (FePro) complexes. Magnetically labeled genetically transformed cells were administered intravenously in tumor bearing mice when tumors reached 0.5 cm in the largest dimension. MRI and single photon emission computed tomography (SPECT) images were acquired 3 and 7 days after cell injection, with a 7 Tesla animal MRI system and a custom built micro-SPECT using Tc-99m, respectively. Expression of hNIS in accumulated cells was determined by staining with anti-hNIS antibody. APCs were efficiently labeled with ferumoxide-protamine sulfate (FePro) complexes and transduced with hNIS gene. Our study showed not only the accumulation of intravenously administered genetically transformed, magnetically labeled APCs in the implanted breast cancer, but also the expression of hNIS gene at the tumor site. Tc-99m activity ratio (tumor/non-tumor) was significantly different between animals that received non-transduced and transduced cells (P < 0.001).
conclusionThis study indicates that genetically transformed, magnetically labeled APCs can be used both as delivery vehicles and cellular probes for detecting in vivo migration and homing of cells. Furthermore, they can potentially be used as a gene carrier system for the treatment of tumor or other diseases.
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