ArticleInternational journal of molecular sciences2008
DNA mimics for the rapid identification of microorganisms by fluorescence in situ hybridization (FISH).
Article in International journal of molecular sciences, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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Who cites it
42 citing papers in PubMed, 108 citations in OpenAlex.
- Peptide Nucleic Acid-Fluorescence In Situ Hybridization (PNA-FISH) for the Detection and Spatial Location of Microorganisms.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Current Analytical Methods and Challenges for the Clinical Diagnosis of Invasive Pulmonary Aspergillosis Infection.Journal of fungi (Basel, Switzerland) · 2024Review
- Ecology ofBiofilm · 2024Review
- Illuminating the oral microbiome and its host interactions: tools and approaches for molecular ecological studies.FEMS microbiology reviews · 2023Review
- Biofilm-Associated Infections in Chronic Wounds and Their Management.Advances in experimental medicine and biology · 2023Article
- Imaging biofilms using fluorescenceFrontiers in cellular and infection microbiology · 2023Review
- Development of a Novel Peptide Nucleic Acid Probe for the Detection ofMicroorganisms · 2022Article
- Advances in the Sensing and Treatment of Wound Biofilms.Angewandte Chemie (Weinheim an der Bergstrasse, Germany) · 2022Review
- Advances in the Sensing and Treatment of Wound Biofilms.Angewandte Chemie (International ed. in English) · 2022Review
- Potential of Flow Cytometric Approaches for Rapid Microbial Detection and Characterization in the Food Industry-A Review.Foods (Basel, Switzerland) · 2021Review
- The Two Weapons against Bacterial Biofilms: Detection and Treatment.Antibiotics (Basel, Switzerland) · 2021Review
- [Research advances on the formation mechanism and diagnosis of bacteria biofilms in chronic wounds].Zhonghua shao shang za zhi = Zhonghua shaoshang zazhi = Chinese journal of burns · 2021Article
- An Introduction to Fluorescence in situ Hybridization in Microorganisms.Methods in molecular biology (Clifton, N.J.) · 2021Article
- A New PNA-FISH Probe TargetingFrontiers in cellular and infection microbiology · 2021Article
- Current Methods, Common Practices, and Perspectives in Tracking and Monitoring Bioinoculants in Soil.Frontiers in microbiology · 2021Review
- Bioinformatic Tools and Guidelines for the Design of Fluorescence In Situ Hybridization Probes.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Application of Nucleic Acid Mimics in Fluorescence In Situ Hybridization.Methods in molecular biology (Clifton, N.J.) · 2021Article
- FISH Variants.Methods in molecular biology (Clifton, N.J.) · 2021Review
- Establishment of a New PNA-FISH Method forMicroorganisms · 2020Article
- FISH and chips: a review of microfluidic platforms for FISH analysis.Medical microbiology and immunology · 2020Review
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Fluorescence in situ hybridization (FISH) is a well-established technique that is used for a variety of purposes, ranging from pathogen detection in clinical diagnostics to the determination of chromosomal stability in stem cell research. The key step of FISH involves the detection of a nucleic acid region and as such, DNA molecules have typically been used to probe for the sequences of interest. However, since the turn of the century, an increasing number of laboratories have started to move on to the more robust DNA mimics methods, most notably peptide and locked nucleic acids (PNA and LNA). In this review, we will cover the state-of-the-art of the different DNA mimics in regard to their application as efficient markers for the presence of individual microbial cells, and consider their potential advantages and pitfalls. Available PNA probes are then reassessed in terms of sensitivity and specificity using rRNA databases. In addition, we also attempt to predict the applicability of DNA mimics in well-known techniques attempting to detect in situ low number of copies of specific nucleic acid sequences such as catalyzed reporter deposition (CARD) and recognition of individual genes (RING) FISH.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.