ArticleBiophysical journal1991
Measurement of mass transport and reaction parameters in bulk solution using photobleaching. Reaction limited binding regime.
Article in Biophysical journal, 1991. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 43 citations in OpenAlex.
- Inferences from FRAP data are model dependent: A subdiffusive analysis.Biophysical journal · 2022Article
- Review
- Single- and two-photon fluorescence recovery after photobleaching.Cold Spring Harbor protocols · 2015Article
- Delivery of molecular and cellular medicine to solid tumors.Advanced drug delivery reviews · 2012Article
- Article
- Simultaneous measurement of anisotropic solute diffusivity and binding reaction rates in biological tissues by FRAP.Annals of biomedical engineering · 2011Article
- Surface charge- and space-dependent transport of proteins in crowded environments of nanotailored posts.ACS nano · 2010Article
- Concentration gradient immunoassay. 2. Computational modeling for analysis and optimization.Analytical chemistry · 2007Article
- Analysis of binding at a single spatially localized cluster of binding sites by fluorescence recovery after photobleaching.Biophysical journal · 2006Article
- Analysis of binding reactions by fluorescence recovery after photobleaching.Biophysical journal · 2004Article
- Fluorescence recovery after photobleaching: a versatile tool for mobility and interaction measurements in pharmaceutical research.Pharmaceutical research · 1999Review
- Theory for measuring bivalent surface binding kinetics using total internal reflection with fluorescence photobleaching recovery.Biophysical journal · 1994Article
- Fluorescence photobleaching with spatial Fourier analysis: measurement of diffusion in light-scattering media.Biophysical journal · 1993Article
- 1995 Whitaker Lecture: delivery of molecules, particles, and cells to solid tumors.Annals of biomedical engineeringReview
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Fluorescence recovery after photobleaching (FRAP) has been used previously to investigate the kinetics of binding to biological surfaces. The present study adapts and further develops this technique for the quantification of mass transport and reaction parameters in bulk media. The technique's ability to obtain the bulk diffusion coefficient, concentration of binding sites, and equilibrium binding constant for ligand/receptor interactions in the reaction limited binding regime is assessed using the B72.3/TAG-72 monoclonal antibody/tumor associated antigen interaction as a model in vitro system. Measurements were independently verified using fluorometry. The bulk diffusion coefficient, concentration of binding sites and equilibrium binding constant for the system investigated were 6.1 +/- 1.1 x 10(-7) cm2/s, 4.4 +/- 0.6 x 10(-7) M, and 2.5 +/- 1.6 x 10(7) M-1, respectively. Model robustness and the applicability of the technique for in vivo quantification of mass transport and reaction parameters are addressed. With a suitable animal model, it is believed that this technique is capable of quantifying mass transport and reaction parameters in vivo.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.