ArticleRNA (New York, N.Y.)2009
Assembly and mobility of exon-exon junction complexes in living cells.
Article in RNA (New York, N.Y.), 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
27 citing papers in PubMed, 43 citations in OpenAlex.
- ORF57/MTA in KSHV Biology and Pathogenesis: Update From 2015.Journal of medical virology · 2026Review
- Article
- Nuclear speckleopathies: developmental disorders caused by variants in genes encoding nuclear speckle proteins.Human genetics · 2024Review
- Article
- Review
- Functional Analysis of Human Hub Proteins and Their Interactors Involved in the Intrinsic Disorder-Enriched Interactions.International journal of molecular sciences · 2017Article
- The exon junction complex factor Y14 is dynamic in the nucleus of the beetleMolecular cytogenetics · 2017Article
- Splicing of Friend Murine Leukemia Virus env-mRNA Enhances Its Ability to Form Polysomes.Frontiers in microbiology · 2016Article
- Dynamic properties of independent chromatin domains measured by correlation spectroscopy in living cells.Epigenetics & chromatin · 2016Article
- Intranuclear binding in space and time of exon junction complex and NXF1 to premRNPs/mRNPs in vivo.The Journal of cell biology · 2015Article
- High-throughput fluorescence correlation spectroscopy enables analysis of proteome dynamics in living cells.Nature biotechnology · 2015Article
- Stability of structured Kaposi's sarcoma-associated herpesvirus ORF57 protein is regulated by protein phosphorylation and homodimerization.Journal of virology · 2015Article
- Comparative assessment of fluorescent transgene methods for quantitative imaging in human cells.Molecular biology of the cell · 2014Article
- Mechanisms of HsSAS-6 assembly promoting centriole formation in human cells.The Journal of cell biology · 2014Article
- Imaging of endogenous messenger RNA splice variants in living cells reveals nuclear retention of transcripts inaccessible to nonsense-mediated decay in Arabidopsis.The Plant cell · 2014Article
- Stable assembly of HIV-1 export complexes occurs cotranscriptionally.RNA (New York, N.Y.) · 2014Article
- Determinants of robustness in spindle assembly checkpoint signalling.Nature cell biology · 2013Article
- The nuclear protein ALY binds to and modulates the activity of transcription factor E2F2.Molecular & cellular proteomics : MCP · 2013Article
- An immunocytochemical study of interchromatin granule clusters in early mouse embryos.BioMed research international · 2013Article
- Interferon-induced antiviral protein MxA interacts with the cellular RNA helicases UAP56 and URH49.The Journal of biological chemistry · 2011Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 2 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The exon-exon junction complex (EJC) forms via association of proteins during splicing of mRNA in a defined manner. Its organization provides a link between biogenesis, nuclear export, and translation of the transcripts. The EJC proteins accumulate in nuclear speckles alongside most other splicing-related factors. We followed the establishment of the EJC on mRNA by investigating the mobility and interactions of a representative set of EJC factors in vivo using a complementary analysis with different fluorescence fluctuation microscopy techniques. Our observations are compatible with cotranscriptional binding of the EJC protein UAP56 confirming that it is involved in the initial phase of EJC formation. RNPS1, REF/Aly, Y14/Magoh, and NXF1 showed a reduction in their nuclear mobility when complexed with RNA. They interacted with nuclear speckles, in which both transiently and long-term immobilized factors were identified. The location- and RNA-dependent differences in the mobility between factors of the so-called outer shell and inner core of the EJC suggest a hypothetical model, in which mRNA is retained in speckles when EJC outer-shell factors are missing.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.