ArticleThe Journal of biological chemistry2009
Membrane permeabilization by trypanosome lytic factor, a cytolytic human high density lipoprotein.
Article in The Journal of biological chemistry, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 40 citations in OpenAlex.
- Partially Replacing Dietary Carbohydrate With Unsaturated Fat or Protein Shifts Protein-Based HDL Subspecies Toward Lower Coronary Heart Disease Risk.Arteriosclerosis, thrombosis, and vascular biology · 2026Trial
- APOL1-mediated kidney disease: a narrative review of the lessons learnt from the past 15 years.BMC nephrology · 2025Review
- Alterations in plasma membrane ion channel structures stimulate NLRP3 inflammasome activation in APOL1 risk milieu.The FEBS journal · 2020Article
- Serum amyloid A exhibits pH dependent antibacterial action and contributes to host defense againstThe Journal of biological chemistry · 2020Article
- APOL1 and kidney cell function.American journal of physiology. Renal physiology · 2019Review
- APOL1: The Balance Imposed by Infection, Selection, and Kidney Disease.Trends in molecular medicine · 2018Review
- Decoding the network of Trypanosoma brucei proteins that determines sensitivity to apolipoprotein-L1.PLoS pathogens · 2018Article
- Apolipoprotein L1 confers pH-switchable ion permeability to phospholipid vesicles.The Journal of biological chemistry · 2017Article
- The Cell Biology of APOL1.Seminars in nephrology · 2017Review
- A Primate APOL1 Variant That Kills Trypanosoma brucei gambiense.PLoS neglected tropical diseases · 2016Article
- Trypanosome Lytic Factor-1 Initiates Oxidation-stimulated Osmotic Lysis of Trypanosoma brucei brucei.The Journal of biological chemistry · 2016Article
- Immune Evasion Strategies of Trypanosoma brucei within the Mammalian Host: Progression to Pathogenicity.Frontiers in immunology · 2016Review
- Large multiallelic copy number variations in humans.Nature genetics · 2015Article
- A retained secretory signal peptide mediates high density lipoprotein (HDL) assembly and function of haptoglobin-related protein.The Journal of biological chemistry · 2014Article
- The molecular arms race between African trypanosomes and humans.Nature reviews. Microbiology · 2014Review
- Trypanosome resistance to human innate immunity: targeting Achilles' heel.Trends in parasitology · 2012Review
- Human apolipoprotein L1 (ApoL1) in cancer and chronic kidney disease.FEBS letters · 2012Review
- APOL1 localization in normal kidney and nondiabetic kidney disease.Journal of the American Society of Nephrology : JASN · 2011Article
- Antimicrobial peptide killing of African trypanosomes.Parasite immunology · 2011Review
- Mechanism of Trypanosoma brucei gambiense (group 1) resistance to human trypanosome lytic factor.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Trypanosome lytic factor (TLF) is a subclass of human high density lipoprotein (HDL) that mediates an innate immune killing of certain mammalian trypanosomes, most notably Trypanosoma brucei brucei, the causative agent of a wasting disease in cattle. Mechanistically, killing is initiated in the lysosome of the target trypanosome where the acidic pH facilitates a membrane-disrupting activity by TLF. Here we utilize a model liposome system to characterize the membrane binding and permeabilizing activity of TLF and its protein constituents, haptoglobin-related protein (Hpr), apolipoprotein L-1 (apoL-1), and apolipoprotein A-1 (apoA-1). We show that TLF efficiently binds and permeabilizes unilamellar liposomes at lysosomal pH, whereas non-lytic human HDL exhibits inefficient permeabilizing activity. Purified, delipidated Hpr and apoL-1 both efficiently permeabilize lipid bilayers at low pH. Trypanosome lytic factor, apoL-1, and apoA-1 exhibit specificity for anionic membranes, whereas Hpr permeabilizes both anionic and zwitterionic membranes. Analysis of the relative particle sizes of susceptible liposomes reveals distinctly different membrane-active behavior for native TLF and the delipidated protein components. We propose that lysosomal membrane damage in TLF-susceptible trypanosomes is initiated by the stable association of the TLF particle with the lysosomal membrane and that this is a property unique to this subclass of human HDL.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.