ReviewJournal of the Royal Society, Interface2009
You're one in a googol: optimizing genes for protein expression.
Review in Journal of the Royal Society, Interface, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
45 citing papers in PubMed.
- Codon-optimization in gene therapy: promises, prospects and challenges.Frontiers in bioengineering and biotechnology · 2024Review
- Genes for highly abundant proteins in Escherichia coli avoid 5' codons that promote ribosomal initiation.PLoS computational biology · 2023Article
- Design of typical genes for heterologous gene expression.Scientific reports · 2022Article
- Tailoring Codon Usage to the Underlying Biology for Protein Expression Optimization.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Optimizing the dynamics of protein expression.Scientific reports · 2019Article
- The effects of codon usage on the formation of secondary structures of nucleocapsid protein of peste des petits ruminants virus.Genes & genomics · 2018Article
- Biochemical mechanisms determine the functional compatibility of heterologous genes.Nature communications · 2018Article
- The Road from Host-Defense Peptides to a New Generation of Antimicrobial Drugs.Molecules (Basel, Switzerland) · 2018Review
- New Vaccine Formulations Containing a Modified Version of the Amastigote 2 Antigen and the Non-VirulentFrontiers in immunology · 2018Article
- Selecting an appropriate method for expressingBiotechnology reports (Amsterdam, Netherlands) · 2017Article
- Stable In Vivo Transgene Expression in Endothelial Cells with Helper-Dependent Adenovirus: Roles of Promoter and Interleukin-10.Human gene therapy · 2017Article
- Gene design, fusion technology and TEV cleavage conditions influence the purification of oxidized disulphide-rich venom peptides in Escherichia coli.Microbial cell factories · 2017Article
- Improving heterologous membrane protein production in Escherichia coli by combining transcriptional tuning and codon usage algorithms.PloS one · 2017Article
- Incorporation of a tag helps to overcome expression variability in a recombinant host.Biotechnology reports (Amsterdam, Netherlands) · 2016Article
- Rationally reduced libraries for combinatorial pathway optimization minimizing experimental effort.Nature communications · 2016Article
- Codon Bias as a Means to Fine-Tune Gene Expression.Molecular cell · 2015Review
- Protein purification using PDZ affinity chromatography.Current protocols in protein science · 2015Article
- Article
- Codon optimization and factorial screening for enhanced soluble expression of human ciliary neurotrophic factor in Escherichia coli.BMC biotechnology · 2014Article
- A critical analysis of codon optimization in human therapeutics.Trends in molecular medicine · 2014Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A vast number of different nucleic acid sequences can all be translated by the genetic code into the same amino acid sequence. These sequences are not all equally useful however; the exact sequence chosen can have profound effects on the expression of the encoded protein. Despite the importance of protein-coding sequences, there has been little systematic study to identify parameters that affect expression. This is probably because protein expression has largely been tackled on an ad hoc basis in many independent projects: once a sequence has been obtained that yields adequate expression for that project, there is little incentive to continue work on the problem. Synthetic biology may now provide the impetus to transform protein expression folklore into design principles, so that DNA sequences may easily be designed to express any protein in any system. In this review, we offer a brief survey of the literature, outline the major challenges in interpreting existing data and constructing robust design algorithms, and propose a way to proceed towards the goal of rational sequence engineering.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.