ArticleJournal of the Royal Society, Interface2010
Going beyond histology. Synchrotron micro-computed tomography as a methodology for biological tissue characterization: from tissue morphology to individual cells.
Article in Journal of the Royal Society, Interface, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
24 citing papers in PubMed.
- Exploration of changes in spatial chondrocyte organisation in human osteoarthritic cartilage by means of 3D imaging.Scientific reports · 2021Trial
- For your eyes too: a new set of images of biological materials from the scientific commissioning of the MOGNO beamline at Sirius.Journal of synchrotron radiation · 2026Article
- In Situ Loading and Time-Resolved Synchrotron-Based Phase Contrast Tomography for the Mechanical Investigation of Connective Knee Tissues: A Proof-of-Concept Study.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Article
- Vibrational spectroscopy identifies myocardial chemical modifications in heart failure with preserved ejection fraction.Journal of translational medicine · 2023Article
- A New Era of Morphological Investigations: Reviewing Methods for Comparative Anatomical Studies.Integrative organismal biology (Oxford, England) · 2023Review
- Synchrotron X-ray biosample imaging: opportunities and challenges.Biophysical reviews · 2022Article
- Micro-computed tomography utility for estimation of intraparenchymal spinal cord cystic lesions in small animals.Neural regeneration research · 2021Article
- A quality optimization approach to image Achilles tendon microstructure by phase-contrast enhanced synchrotron micro-tomography.Scientific reports · 2021Article
- Cardiac multi-scale investigation of the right and left ventricleCardiovascular diagnosis and therapy · 2020Review
- Article
- Ex vivo evaluation of an atherosclerotic human coronary artery via histology and high-resolution hard X-ray tomography.Scientific reports · 2019Article
- Visualisation of HER2 homodimers in single cells from HER2 overexpressing primary formalin fixed paraffin embedded tumour tissue.Molecular medicine (Cambridge, Mass.) · 2019Article
- A quantitative analysis of 3D-cell distribution in regenerating muscle-skeletal system with synchrotron X-ray computed microtomography.Scientific reports · 2018Article
- In vitro method for 3D morphometry of human articular cartilage chondrons based on micro-computed tomography.Osteoarthritis and cartilage · 2018Article
- Hydrogels as a Replacement Material for Damaged Articular Hyaline Cartilage.Materials (Basel, Switzerland) · 2016Review
- New trends in articular cartilage repair.Journal of experimental orthopaedics · 2015Article
- Automatic analysis of the 3-D microstructure of fruit parenchyma tissue using X-ray micro-CT explains differences in aeration.BMC plant biology · 2015Article
- Three-dimensional imaging of human stem cells using soft X-ray tomography.Journal of the Royal Society, Interface · 2015Article
- [Prostaglandin E₂: innovative approaches for tissue engineering of articular cartilage].Der Pathologe · 2014Review
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Current light microscopic methods such as serial sectioning, confocal microscopy or multiphoton microscopy are severely limited in their ability to analyse rather opaque biological structures in three dimensions, while electron optical methods offer either a good three-dimensional topographic visualization (scanning electron microscopy) or high-resolution imaging of very thin samples (transmission electron microscopy). However, sample preparation commonly results in a significant alteration and the destruction of the three-dimensional integrity of the specimen. Depending on the selected photon energy, the interaction between X-rays and biological matter provides semi-transparency of the specimen, allowing penetration of even large specimens. Based on the projection-slice theorem, angular projections can be used for tomographic imaging. This method is well developed in medical and materials science for structure sizes down to several micrometres and is considered as being non-destructive. Achieving a spatial and structural resolution that is sufficient for the imaging of cells inside biological tissues is difficult due to several experimental conditions. A major problem that cannot be resolved with conventional X-ray sources are the low differences in density and absorption contrast of cells and the surrounding tissue. Therefore, X-ray monochromatization coupled with a sufficiently high photon flux and coherent beam properties are key requirements and currently only possible with synchrotron-produced X-rays. In this study, we report on the three-dimensional morphological characterization of articular cartilage using synchrotron-generated X-rays demonstrating the spatial distribution of single cells inside the tissue and their quantification, while comparing our findings to conventional histological techniques.
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