ArticleMicrovascular research2009
Inhibition of angiogenesis in vitro: a central role for beta-actin dependent cytoskeletal remodeling.
Article in Microvascular research, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
16 citing papers in PubMed, 20 citations in OpenAlex.
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- The Role of Nuclear Actin in Genome Organization and Gene Expression Regulation During Differentiation.Results and problems in cell differentiation · 2022Review
- Crotoxin Inhibits Endothelial Cell Functions in Two- and Three-dimensional Tumor Microenvironment.Frontiers in pharmacology · 2021Article
- Crotoxin promotes macrophage reprogramming towards an antiangiogenic phenotype.Scientific reports · 2019Article
- β-Actin: Not a Suitable Internal Control of Hepatic Fibrosis Caused byFrontiers in microbiology · 2019Article
- Long-term gene therapy with Del1 fragment using nonviral vectors in mice with explanted tumors.OncoTargets and therapy · 2016Article
- Pericyte chemomechanics and the angiogenic switch: insights into the pathogenesis of proliferative diabetic retinopathy?Investigative ophthalmology & visual science · 2015Article
- Pigment epithelium-derived factor mediates impaired lung vascular development in neonatal hyperoxia.American journal of respiratory cell and molecular biology · 2015Article
- Pericyte contractility controls endothelial cell cycle progression and sprouting: insights into angiogenic switch mechanics.American journal of physiology. Cell physiology · 2014Article
- Microvascular complications and diabetic retinopathy: recent advances and future implications.Future medicinal chemistry · 2013Review
- Human platelet-rich plasma- and extracellular matrix-derived peptides promote impaired cutaneous wound healing in vivo.PloS one · 2012Article
- Microvascular modifications in diabetic retinopathy.Current diabetes reports · 2011Review
- Calpain- and talin-dependent control of microvascular pericyte contractility and cellular stiffness.Microvascular research · 2010Article
- Article
- Bioactive peptides derived from vascular endothelial cell extracellular matrices promote microvascular morphogenesis and wound healing in vitro.Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair SocietyArticle
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Endothelial cell migration and proliferation, central steps in both physiologic and pathologic angiogenesis, require cytoskeletal-dependent remodeling, which is, in large part, achieved by the dynamic regulation of the beta-actin network. Specifically, the beta-actin network has previously been shown to be (i) enriched in regions of highly motile cytoplasm, and (ii) modulated by its isoactin-specific barbed-end capping protein, beta cap73. We hypothesize that regulated over-expression of beta cap73 could disrupt angiogenesis by capping beta-actin-filament assembly thus inhibiting the incipient cellular migration and microvascular morphogenesis that ensues. Indeed, upon infection of capillary endothelial cells (cEC) with an adenovirus encoding the full-length beta cap73 (Ad-beta cap73), there is a robust cellular rounding response that occurs concomitantly with cytoskeletal disruption, as visualized with immunofluorescence microscopy. Further, we demonstrate that over-expression of Ad-beta cap73 inhibits cEC migration in wound healing studies. Quantitative in vitro angiogenesis assays reveal that Ad-beta cap73 not only prevents endothelial cells from forming capillary-like networks, but also induces the collapse of preformed endothelial tubes. In testing whether Ad-beta cap73 impairs angiogenic events by inducing anoikis/apoptosis, we demonstrate that beta cap73 infection activates a caspase-3-mediated cell death response as observed by quantitative Western blotting and immunofluorescence analyses. Altogether, these findings suggest that endothelial-specific targeting and beta cap73 over-expression may represent an innovative therapeutic approach capable of abrogating pathologic angiogenesis.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.