ArticleBMC molecular biology2009
Evidence of a tick RNAi pathway by comparative genomics and reverse genetics screen of targets with known loss-of-function phenotypes in Drosophila.
Article in BMC molecular biology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
35 citing papers in PubMed, 80 citations in OpenAlex.
- Biogenic nanomaterials: A green strategy for sustainable vector-borne disease management.Pest management science · 2026Review
- Hl48 modulates argonaute 2 to enhance RNA interference in ticks.Frontiers in cellular and infection microbiology · 2026Article
- Vectorial dynamics underpinning current and future tick-borne virus emergence in Europe.The Journal of general virology · 2024Review
- Ixodes ricinus as potential vector for Usutu virus.PLoS neglected tropical diseases · 2024Article
- RNA activation in ticks.Scientific reports · 2023Article
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- Pyrokinin receptor silencing in females of the southern cattle tick Rhipicephalus (Boophilus) microplus is associated with a reproductive fitness cost.Parasites & vectors · 2022Article
- Article
- Potential Mechanisms of Transmission of Tick-Borne Viruses at the Virus-Tick Interface.Frontiers in microbiology · 2022Review
- Antiviral RNA interference in disease vector (Asian longhorned) ticks.PLoS pathogens · 2021Article
- Induced Transient Immune Tolerance in Ticks and Vertebrate Host: A Keystone of Tick-Borne Diseases?Frontiers in immunology · 2021Review
- Identification of African swine fever virus-like elements in the soft tick genome provides insights into the virus' evolution.BMC biology · 2020Article
- Article
- Key Mechanistic Principles and Considerations Concerning RNA Interference.Frontiers in plant science · 2020Review
- RNAi mediated knockdown of RpL11, RpS2, and tra-2 led to reduced reproduction of Phytoseiulus persimilis.Experimental & applied acarology · 2019Article
- The Tick Cell Biobank: A global resource for in vitro research on ticks, other arthropods and the pathogens they transmit.Ticks and tick-borne diseases · 2018Review
- Liposome mediated double-stranded RNA delivery to silence ribosomal protein P0 in the tick Rhipicephalus haemaphysaloides.Ticks and tick-borne diseases · 2018Article
- Rewired RNAi-mediated genome surveillance in house dust mites.PLoS genetics · 2018Article
- Dendritic transport of tick-borne flavivirus RNA by neuronal granules affects development of neurological disease.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Ixodes scapularis and Ixodes ricinus tick cell lines respond to infection with tick-borne encephalitis virus: transcriptomic and proteomic analysis.Parasites & vectors · 2015Article
Corrections and comments
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Authors and funding
9 authors at 5 institutions in 2 countries.
Funding
Abstract
backgroundThe Arthropods are a diverse group of organisms including Chelicerata (ticks, mites, spiders), Crustacea (crabs, shrimps), and Insecta (flies, mosquitoes, beetles, silkworm). The cattle tick, Rhipicephalus (Boophilus) microplus, is an economically significant ectoparasite of cattle affecting cattle industries world wide. With the availability of sequence reads from the first Chelicerate genome project (the Ixodes scapularis tick) and extensive R. microplus ESTs, we investigated evidence for putative RNAi proteins and studied RNA interference in tick cell cultures and adult female ticks targeting Drosophila homologues with known cell viability phenotype.
resultsWe screened 13,643 R. microplus ESTs and I. scapularis genome reads to identify RNAi related proteins in ticks. Our analysis identified 31 RNAi proteins including a putative tick Dicer, RISC associated (Ago-2 and FMRp), RNA dependent RNA polymerase (EGO-1) and 23 homologues implicated in dsRNA uptake and processing. We selected 10 R. microplus ESTs with >80% similarity to D. melanogaster proteins associated with cell viability for RNAi functional screens in both BME26 R. microplus embryonic cells and female ticks in vivo. Only genes associated with proteasomes had an effect on cell viability in vitro. In vivo RNAi showed that 9 genes had significant effects either causing lethality or impairing egg laying.
conclusionWe have identified key RNAi-related proteins in ticks and along with our loss-of-function studies support a functional RNAi pathway in R. microplus. Our preliminary studies indicate that tick RNAi pathways may differ from that of other Arthropods such as insects.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.