ArticleDevelopmental dynamics : an official publication of the American Association of Anatomists2009
Transient and transgenic analysis of the zebrafish ventricular myosin heavy chain (vmhc) promoter: an inhibitory mechanism of ventricle-specific gene expression.
Article in Developmental dynamics : an official publication of the American Association of Anatomists, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 33 citations in OpenAlex.
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- Control of cardiac contractions using Cre-lox and degron strategies in zebrafish.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Neuron navigator 3 (NAV3) is required for heart development in zebrafish.Fish physiology and biochemistry · 2022Article
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- Hemodynamic Forces Regulate Cardiac Regeneration-Responsive Enhancer Activity during Ventricle Regeneration.International journal of molecular sciences · 2021Article
- A modifier screen identifiesJCI insight · 2016Article
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- An early requirement for nkx2.5 ensures the first and second heart field ventricular identity and cardiac function into adulthood.Developmental biology · 2015Article
- Understanding cardiac sarcomere assembly with zebrafish genetics.Anatomical record (Hoboken, N.J. : 2007) · 2014Review
- Expression of tropomyosin in relation to myofibrillogenesis in axolotl hearts.Regenerative medicine research · 2013Review
- Nkx genes are essential for maintenance of ventricular identity.Development (Cambridge, England) · 2013Article
- Lrrc10 is a novel cardiac-specific target gene of Nkx2-5 and GATA4.Journal of molecular and cellular cardiology · 2013Article
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- α-Actinin2 is required for the lateral alignment of Z discs and ventricular chamber enlargement during zebrafish cardiogenesis.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2012Article
- Development of a transient expression assay for detecting environmental oestrogens in zebrafish and medaka embryos.BMC biotechnology · 2012Article
- Depletion of zebrafish Tcap leads to muscular dystrophy via disrupting sarcomere-membrane interaction, not sarcomere assembly.Human molecular genetics · 2009Article
- Promoter analysis of ventricular myosin heavy chain (vmhc) in zebrafish embryos.Developmental dynamics : an official publication of the American Association of Anatomists · 2009Article
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Authors and funding
2 authors at 1 institution in 1 country.
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Abstract
The zebrafish ventricular myosin heavy chain (vmhc) gene exhibits restricted expression in the ventricle. However, the molecular mechanism underlying this chamber-specific expression is unclear. Here, we exploited both transient and transgenic technologies to dissect the zebrafish vmhc promoter. We demonstrated that a combination of two transient assays in this animal model quickly identified chamber-specific cis-elements, isolating a 2.2 kb fragment upstream from the vmhc gene that can drive ventricle-specific expression. Furthermore, deletion analysis identified multiple cis-elements that exhibited cardiac-specific expression. To achieve chamber specificity, a distal element was required to coordinate with and suppress a proximal enhancer element. Finally, we discovered that Nkx2.5-binding sites (NKE) were essential for this repressive function. In summary, our study of the zebrafish vmhc promoter suggests that ventricle-specific expression is achieved through an inhibitory mechanism that suppresses expression in the atrium. Developmental Dynamics 238:1564-1573, 2009. (c) 2009 Wiley-Liss, Inc.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.