ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2009
Raphé neurons stimulate respiratory circuit activity by multiple mechanisms via endogenously released serotonin and substance P.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 174 papers.
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Who cites it
174 citing papers in PubMed, 259 citations in OpenAlex.
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- The Role of Loss-of-Function KCNH2 Variants in Cardiac Arrhythmias, Seizures and the Risk of Sudden Unexpected Death in Epilepsy.Journal of neurochemistry · 2026Review
- Serotonin Associated Mechanisms in Sudden Unexpected Death in Epilepsy: A Review.Revista de neurologia · 2026Review
- Dysregulated Plasticity in Serotonin, Galanin, and Opioid Systems Contributes to Limbic Seizure Recruitment in Wistar Audiogenic Rat.Journal of neurochemistry · 2026Article
- Tachykinergic signaling mediated by NK1 receptors in the respiratory parafacial region drives respiratory output and contributes to the chemoreflex response to COPflugers Archiv : European journal of physiology · 2026Article
- Integrated single-cell atlases unveil the operation principles of whole-brain 5-HT neuronal subsystems.Science advances · 2025Article
- Altered Interictal Bed Nucleus of Stria Terminalis Connectivity in Patients With Temporal Lobe Epilepsy.Neurology · 2025Article
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- Lethal Interactions of neuronal networks in epilepsy mediated by both synaptic and volume transmission indicate approaches to prevention.Progress in neurobiology · 2025Review
- A jack of all trades: Hermaphrodite-specific serotonergic neuron inJournal of biosciences · 2025Article
- Neuropeptide and serotonin co-transmission sets the activity pattern in the C. elegans egg-laying circuit.Current biology : CB · 2024Article
- Hypoxia evokes a sequence of raphe-pontomedullary network operations for inspiratory drive amplification and gasping.Journal of neurophysiology · 2024Article
- Intrinsic Molecular Proton Sensitivity Underlies GPR4 Effects on Retrotrapezoid Nucleus Neuronal Activation and COThe Journal of neuroscience : the official journal of the Society for Neuroscience · 2024Article
- Human IPSC-Derived PreBötC-Like Neurons and Development of an Opiate Overdose and Recovery Model.Advanced biology · 2024Article
- Review
- Galaninergic and hypercapnia-activated neuronal projections to the ventral respiratory column.Brain structure & function · 2024Article
- Impact of Serotonergic 5HTInternational journal of molecular sciences · 2024Article
- Serotonin therapies for opioid-induced disordered swallow and respiratory depression.Journal of applied physiology (Bethesda, Md. : 1985) · 2024Article
- Inspiratory and sigh breathing rhythms depend on distinct cellular signalling mechanisms in the preBötzinger complex.The Journal of physiology · 2024Article
114 more citing papers are in PubMed but not listed here.
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
Brainstem serotonin (5-HT) neurons modulate activity of many neural circuits in the mammalian brain, but in many cases endogenous mechanisms have not been resolved. Here, we analyzed actions of raphé 5-HT neurons on respiratory network activity including at the level of the pre-Bötzinger complex (pre-BötC) in neonatal rat medullary slices in vitro, and in the more intact nervous system of juvenile rats in arterially perfused brainstem-spinal cord preparations in situ. At basal levels of activity, excitation of the respiratory network via simultaneous release of 5-HT and substance P (SP), acting at 5-HT(2A/2C), 5-HT(4), and/or neurokinin-1 receptors, was required to maintain inspiratory motor output in both the neonatal and juvenile systems. The midline raphé obscurus contained spontaneously active 5-HT neurons, some of which projected to the pre-BötC and hypoglossal motoneurons, colocalized 5-HT and SP, and received reciprocal excitatory connections from the pre-BötC. Experimentally augmenting raphé obscurus activity increased motor output by simultaneously exciting pre-BötC and motor neurons. Biophysical analyses in vitro demonstrated that 5-HT and SP modulated background cation conductances in pre-BötC and motor neurons, including a nonselective cation leak current that contributed to the resting potential, which explains the neuronal depolarization that augmented motor output. Furthermore, we found that 5-HT, but not SP, can transform the electrophysiological phenotype of some pre-BötC neurons to intrinsic bursters, providing 5-HT with an additional role in promoting rhythm generation. We conclude that raphé 5-HT neurons excite key circuit components required for generation of respiratory motor output.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.